Purpose. To determine if the neuronal protein GAP-43 is expressed in adult retina and if this expression is altered following laser-induced injury. Methods. The retinas of two adult New Zealand Red rabbits were irradiated in a 5 x 10 lesion array with a multiline argon laser (488-514.5 nm, 0.5 mm spot size, 285mW/cm2/7 msec). Eight h later retinas were removed, zinc-fixed, embedded in paraffin, sectioned, and tissue-treated with 50 ug/ml mouse monoclonal anti-GAP-43 present in 0.5% BSA and 0.05% Triton X-100 for 18 h at 4C. Controls received normal mouse serum or mouse monoclonal anti-beta-galactosidase. The sections were then washed, incubated with FITC-labeled goat anti-mouse IgG1 for 1 h at 4C, and examined by fluorescence microscopy. Results. Lesions showed near full thickness retinal pathology. GAP-43 immunostaining consisted of a trilaminar band in the inner plexiform layer of the retina. A punctate pattern was evident within the three lines of fluorescence, suggesting that axon terminals had been labeled. Staining patterns were similar for both laser-irradiated and control areas. Conclusions. GAP-43 immunostaining is intense in the inner plexiform layer where it may be present in the axon terminals of bipolar cells. In rabbit retina, GAP-43 expression is not up-regulated 8 h after laser injury but occurs uniformly throughout the length of the inner plexiform layer.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Feb 15 1996|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience