Signal-mediated nuclear transport in simian virus 40-transformed cells is regulated by large tumor antigen

Carl M. Feldherr, Robert E. Lanford, Debra Akin

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    66 Scopus citations

    Abstract

    Transformation of cultured cells with simian virus 40 (SV40), or transfection with the early region of the SV40 genome, causes a significant increase in both the rate of signal-mediated nuclear transport and the functional size of the transport channels (located in the pore complexes). By microinjecting purified large tumor (T) antigen into the cytoplasm of murine BALB/c 3T3 cells, we have demonstrated that this protein alone can account for the increase in transport capacity. The T antigen-dependent changes can be partially inhibited by cycloheximide and require a functional nuclear localization sequence. Although necessary, the nuclear localization sequence by itself cannot produce the observed variations in nuclear permeability and presumably functions in a "helper" capacity, in association with another, as yet unidentified domain.

    Original languageEnglish (US)
    Pages (from-to)11002-11005
    Number of pages4
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume89
    Issue number22
    DOIs
    StatePublished - 1992

    ASJC Scopus subject areas

    • General

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