Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization

Xianlin Han, Kui Yang, Hua Cheng, Kora N. Fikes, Richard W. Gross

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

This article presents a novel methodology for the analysis of ethanolamine glycerophospholipid (PE) and lysoPE molecular species directly from lipid extracts of biological samples. Through brief treatment of lipid extracts with fluorenylmethoxylcarbonyl (Fmoc) chloride, PE and lysoPE species were selectively derivatized to their corresponding carbamates. The reaction solution was infused directly into the ion source of an electrospray ionization mass spectrometer after appropriate dilution. The facile loss of the Fmoc moiety dramatically enhanced the analytic sensitivity and allowed the identification and quantitation of low-abundance molecular species. A detection limitation of attomoles (amoles) per microliter for PE and lysoPE analysis was readily achieved using this technique (at least a 100-fold improvement from our previous method) with a >15,000-fold dynamic range. Through intrasource separation and multidimensional mass spectrometry array analysis of derivatized species, marked improvements in signal-to-noise ratio, molecular species identification, and quantitation can be realized. The procedure is both simple and effective and can be extended to analyze many other lipid classes or other cellular metabolites by adjustments in specific derivatization conditions. Thus, through judicious derivatization, a new dimension exploiting specific functional reactivities in each lipid class can be used in conjunction with shotgun lipidomics to penetrate farther into the low-abundance regime of cellular lipidomes.

Original languageEnglish (US)
Pages (from-to)1548-1560
Number of pages13
JournalJournal of Lipid Research
Volume46
Issue number7
DOIs
StatePublished - Dec 1 2005
Externally publishedYes

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Firearms
Lipids
Glycerophospholipids
Electrospray ionization
Ethanolamine
Carbamates
Signal-To-Noise Ratio
Mass spectrometers
Ion sources
Metabolites
Dilution
Mass spectrometry
Chlorides
Mass Spectrometry
Signal to noise ratio
Ions
phosphorylethanolamine

Keywords

  • Electrospray ionization-mass spectrometry
  • Fluorenylmethoxylcarbonyl derivatization
  • Lipidome
  • Lipidomics
  • Lysophosphatidylethanolamine
  • Multidimensional mass spectrometry
  • Plasmalogen

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization. / Han, Xianlin; Yang, Kui; Cheng, Hua; Fikes, Kora N.; Gross, Richard W.

In: Journal of Lipid Research, Vol. 46, No. 7, 01.12.2005, p. 1548-1560.

Research output: Contribution to journalArticle

Han, Xianlin ; Yang, Kui ; Cheng, Hua ; Fikes, Kora N. ; Gross, Richard W. / Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization. In: Journal of Lipid Research. 2005 ; Vol. 46, No. 7. pp. 1548-1560.
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