Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction

Wilson C. Fok, Carolina Livi, Alex Bokov, Zhen Yu, Yidong Chen, Arlan Richardson, Viviana I. Pérez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin had essentially no effect on the transcriptome as compared to DR. For example, only six transcripts were significantly altered by rapamycin while mice fed DR showed a significant change in over 1000 transcripts. Using ingenuity pathway analysis, we found that stearate biosynthesis and circadian rhythm signaling were significantly changed by DR. Our findings showing that DR, but not rapamycin, has an effect on the transcriptome of the adipose tissue, suggesting that these two manipulations increase lifespan through different mechanisms/pathways.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalMechanisms of Ageing and Development
Volume140
Issue number1
DOIs
StatePublished - 2014

Fingerprint

White Adipose Tissue
Sirolimus
Transcriptome
Stearates
Microarray Analysis
Circadian Rhythm
Adipose Tissue
Diet
Growth
Pharmaceutical Preparations

Keywords

  • Adipose
  • Dietary restriction
  • Fat
  • Microarrays
  • Rapamycin
  • Transcriptome

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

Cite this

Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. / Fok, Wilson C.; Livi, Carolina; Bokov, Alex; Yu, Zhen; Chen, Yidong; Richardson, Arlan; Pérez, Viviana I.

In: Mechanisms of Ageing and Development, Vol. 140, No. 1, 2014, p. 23-29.

Research output: Contribution to journalArticle

Fok, Wilson C. ; Livi, Carolina ; Bokov, Alex ; Yu, Zhen ; Chen, Yidong ; Richardson, Arlan ; Pérez, Viviana I. / Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. In: Mechanisms of Ageing and Development. 2014 ; Vol. 140, No. 1. pp. 23-29.
@article{cd7c14bace2b4094a59dd2a7e5696373,
title = "Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction",
abstract = "Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin had essentially no effect on the transcriptome as compared to DR. For example, only six transcripts were significantly altered by rapamycin while mice fed DR showed a significant change in over 1000 transcripts. Using ingenuity pathway analysis, we found that stearate biosynthesis and circadian rhythm signaling were significantly changed by DR. Our findings showing that DR, but not rapamycin, has an effect on the transcriptome of the adipose tissue, suggesting that these two manipulations increase lifespan through different mechanisms/pathways.",
keywords = "Adipose, Dietary restriction, Fat, Microarrays, Rapamycin, Transcriptome",
author = "Fok, {Wilson C.} and Carolina Livi and Alex Bokov and Zhen Yu and Yidong Chen and Arlan Richardson and P{\'e}rez, {Viviana I.}",
year = "2014",
doi = "10.1016/j.mad.2014.07.004",
language = "English (US)",
volume = "140",
pages = "23--29",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction

AU - Fok, Wilson C.

AU - Livi, Carolina

AU - Bokov, Alex

AU - Yu, Zhen

AU - Chen, Yidong

AU - Richardson, Arlan

AU - Pérez, Viviana I.

PY - 2014

Y1 - 2014

N2 - Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin had essentially no effect on the transcriptome as compared to DR. For example, only six transcripts were significantly altered by rapamycin while mice fed DR showed a significant change in over 1000 transcripts. Using ingenuity pathway analysis, we found that stearate biosynthesis and circadian rhythm signaling were significantly changed by DR. Our findings showing that DR, but not rapamycin, has an effect on the transcriptome of the adipose tissue, suggesting that these two manipulations increase lifespan through different mechanisms/pathways.

AB - Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin had essentially no effect on the transcriptome as compared to DR. For example, only six transcripts were significantly altered by rapamycin while mice fed DR showed a significant change in over 1000 transcripts. Using ingenuity pathway analysis, we found that stearate biosynthesis and circadian rhythm signaling were significantly changed by DR. Our findings showing that DR, but not rapamycin, has an effect on the transcriptome of the adipose tissue, suggesting that these two manipulations increase lifespan through different mechanisms/pathways.

KW - Adipose

KW - Dietary restriction

KW - Fat

KW - Microarrays

KW - Rapamycin

KW - Transcriptome

UR - http://www.scopus.com/inward/record.url?scp=84906675399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906675399&partnerID=8YFLogxK

U2 - 10.1016/j.mad.2014.07.004

DO - 10.1016/j.mad.2014.07.004

M3 - Article

C2 - 25075714

AN - SCOPUS:84906675399

VL - 140

SP - 23

EP - 29

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 1

ER -