Short-term culture of peritoneum explants confirms attachment of endometrium to intact peritoneal mesothelium

Craig A. Witz, Mika R. Thomas, Iris A. Montoya-Rodriguez, Anitha S. Nair, Victoria E. Centonze, Robert S Schenken

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Objective: To evaluate the initial adhesion of endometrium to the peritoneum. Design: Descriptive study using light and confocal laser-scanning microscopy, immunohistochemistry, and transmission electron microscopy. Setting: University-based laboratory. Patient(s): Women without endometriosis undergoing surgery for benign conditions. Intervention(s): None. Main Outcome Measure(s): Explants of peritoneum (n = 20), prepared from four patients, were cultured for 1 hour with mechanically dispersed proliferative or secretory endometrium. Peritoneum was cultured with endometrium from the same patient. Specimens were fixed and serially sectioned for hematoxylin and eosin stain, immunohistochemistry using an anti-cytokeratin monoclonal antibody, and transmission electron microscopy. Result(s): In 17 of 20 explants, endometrium was adherent to intact mesothelium. There was no evidence of transmesothelial invasion at any sites of attachment. Although in most cases endometrium was adherent to mesothelium via endometrial stroma, there were many sites of endometrial epithelium-mesothelium attachment. Confocal laser scanning microscopy demonstrated an intact monolayer of cytokeratin-positive cells below the sites of endometrial implantation. Transmission electron microscopy demonstrated intact, viable, mesothelial cells below sites of attachment. Conclusion(s): This study demonstrates that endometrium rapidly adheres to intact peritoneal mesothelium. In addition, this study demonstrates that endometrial epithelial cells, as well as stroma, can attach to mesothelium. Further studies are needed that characterize the mechanism of endometrial-mesothelial cell adhesion.

Original languageEnglish (US)
Pages (from-to)385-390
Number of pages6
JournalFertility and Sterility
Volume75
Issue number2
DOIs
StatePublished - 2001

Fingerprint

Peritoneum
Endometrium
Epithelium
Transmission Electron Microscopy
Keratins
Confocal Microscopy
Immunohistochemistry
Endometriosis
Hematoxylin
Eosine Yellowish-(YS)
Cell Adhesion
Coloring Agents
Epithelial Cells
Monoclonal Antibodies
Outcome Assessment (Health Care)
Light

Keywords

  • Confocal
  • Electron microscopy
  • Endometriosis
  • Laser-scanning microscopy
  • Mesothelium
  • Peritoneum

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Short-term culture of peritoneum explants confirms attachment of endometrium to intact peritoneal mesothelium. / Witz, Craig A.; Thomas, Mika R.; Montoya-Rodriguez, Iris A.; Nair, Anitha S.; Centonze, Victoria E.; Schenken, Robert S.

In: Fertility and Sterility, Vol. 75, No. 2, 2001, p. 385-390.

Research output: Contribution to journalArticle

Witz, Craig A. ; Thomas, Mika R. ; Montoya-Rodriguez, Iris A. ; Nair, Anitha S. ; Centonze, Victoria E. ; Schenken, Robert S. / Short-term culture of peritoneum explants confirms attachment of endometrium to intact peritoneal mesothelium. In: Fertility and Sterility. 2001 ; Vol. 75, No. 2. pp. 385-390.
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AU - Nair, Anitha S.

AU - Centonze, Victoria E.

AU - Schenken, Robert S

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AB - Objective: To evaluate the initial adhesion of endometrium to the peritoneum. Design: Descriptive study using light and confocal laser-scanning microscopy, immunohistochemistry, and transmission electron microscopy. Setting: University-based laboratory. Patient(s): Women without endometriosis undergoing surgery for benign conditions. Intervention(s): None. Main Outcome Measure(s): Explants of peritoneum (n = 20), prepared from four patients, were cultured for 1 hour with mechanically dispersed proliferative or secretory endometrium. Peritoneum was cultured with endometrium from the same patient. Specimens were fixed and serially sectioned for hematoxylin and eosin stain, immunohistochemistry using an anti-cytokeratin monoclonal antibody, and transmission electron microscopy. Result(s): In 17 of 20 explants, endometrium was adherent to intact mesothelium. There was no evidence of transmesothelial invasion at any sites of attachment. Although in most cases endometrium was adherent to mesothelium via endometrial stroma, there were many sites of endometrial epithelium-mesothelium attachment. Confocal laser scanning microscopy demonstrated an intact monolayer of cytokeratin-positive cells below the sites of endometrial implantation. Transmission electron microscopy demonstrated intact, viable, mesothelial cells below sites of attachment. Conclusion(s): This study demonstrates that endometrium rapidly adheres to intact peritoneal mesothelium. In addition, this study demonstrates that endometrial epithelial cells, as well as stroma, can attach to mesothelium. Further studies are needed that characterize the mechanism of endometrial-mesothelial cell adhesion.

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