Short infrared (IR) laser pulses can induce nanoporation

Caleb C. Roth, Ronald A. Barnes, Bennett L. Ibey, Randolph D. Glickman, Hope T. Beier

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Short infrared (IR) laser pulses on the order of hundreds of microseconds to single milliseconds with typical wavelengths of 1800-2100 nm, have shown the capability to reversibly stimulate action potentials (AP) in neuronal cells. While the IR stimulation technique has proven successful for several applications, the exact mechanism(s) underlying the AP generation has remained elusive. To better understand how IR pulses cause AP stimulation, we determined the threshold for the formation of nanopores in the plasma membrane. Using a surrogate calcium ion, thallium, which is roughly the same shape and charge, but lacks the biological functionality of calcium, we recorded the flow of thallium ions into an exposed cell in the presence of a battery of channel antagonists. The entry of thallium into the cell indicated that the ions entered via nanopores. The data presented here demonstrate a basic understanding of the fundamental effects of IR stimulation and speculates that nanopores, formed in response to the IR exposure, play an upstream role in the generation of AP.

Original languageEnglish (US)
Title of host publicationClinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
PublisherSPIE
Volume9690
ISBN (Electronic)9781628419603
DOIs
StatePublished - 2016
EventClinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation - San Francisco, United States
Duration: Feb 13 2016Feb 16 2016

Other

OtherClinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
CountryUnited States
CitySan Francisco
Period2/13/162/16/16

Keywords

  • action potential (AP)
  • infrared nerve stimulation
  • lipid nanopore
  • nanoporation
  • thallium

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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