Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast

Caroline Dubacq, Raphaël Guerois, Régis Courbeyrette, Katsumi Kitagawa, Carl Mann

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

Sgt1p is a highly conserved eucaryotic protein that is required for both SCF (Skp1p/Cdc53p-Cullin-F-box)-mediated ubiquitination and kinetochore function in yeast. We show here that Sgt1p is also involved in the cyclic AMP (cAMP) pathway in Saccharomyces cerevisiae. SGT1 is an allele.specific suppressor of cdc35-1, a thermosensitive mutation in the leucine-rich repeat domain of the adenylyl cyclase Cyr1p/Cdc35p. We demonstrate that Sgt1p and Cyr1p/Cdc35p physically interact and that the activity of the cAMP pathway is affected in an sgt1 conditional mutant. Sequence analysis suggests that Sgt1p has features of a cochaperone. Thus, Sgt1p is a novel activator of adenylyl cyclase in S. cerevisiae and may function in the assembly or the conformational activation of specific multiprotein complexes.

Original languageEnglish (US)
Pages (from-to)568-582
Number of pages15
JournalEukaryotic Cell
Volume1
Issue number4
DOIs
StatePublished - Aug 2002

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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