Abstract
Sgt1p is a highly conserved eucaryotic protein that is required for both SCF (Skp1p/Cdc53p-Cullin-F-box)-mediated ubiquitination and kinetochore function in yeast. We show here that Sgt1p is also involved in the cyclic AMP (cAMP) pathway in Saccharomyces cerevisiae. SGT1 is an allele.specific suppressor of cdc35-1, a thermosensitive mutation in the leucine-rich repeat domain of the adenylyl cyclase Cyr1p/Cdc35p. We demonstrate that Sgt1p and Cyr1p/Cdc35p physically interact and that the activity of the cAMP pathway is affected in an sgt1 conditional mutant. Sequence analysis suggests that Sgt1p has features of a cochaperone. Thus, Sgt1p is a novel activator of adenylyl cyclase in S. cerevisiae and may function in the assembly or the conformational activation of specific multiprotein complexes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 568-582 |
| Number of pages | 15 |
| Journal | Eukaryotic Cell |
| Volume | 1 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1 2002 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Molecular Biology
Cite this
Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast. / Dubacq, Caroline; Guerois, Raphaël; Courbeyrette, Régis; Kitagawa, Katsumi; Mann, Carl.
In: Eukaryotic Cell, Vol. 1, No. 4, 01.08.2002, p. 568-582.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast
AU - Dubacq, Caroline
AU - Guerois, Raphaël
AU - Courbeyrette, Régis
AU - Kitagawa, Katsumi
AU - Mann, Carl
PY - 2002/8/1
Y1 - 2002/8/1
N2 - Sgt1p is a highly conserved eucaryotic protein that is required for both SCF (Skp1p/Cdc53p-Cullin-F-box)-mediated ubiquitination and kinetochore function in yeast. We show here that Sgt1p is also involved in the cyclic AMP (cAMP) pathway in Saccharomyces cerevisiae. SGT1 is an allele.specific suppressor of cdc35-1, a thermosensitive mutation in the leucine-rich repeat domain of the adenylyl cyclase Cyr1p/Cdc35p. We demonstrate that Sgt1p and Cyr1p/Cdc35p physically interact and that the activity of the cAMP pathway is affected in an sgt1 conditional mutant. Sequence analysis suggests that Sgt1p has features of a cochaperone. Thus, Sgt1p is a novel activator of adenylyl cyclase in S. cerevisiae and may function in the assembly or the conformational activation of specific multiprotein complexes.
AB - Sgt1p is a highly conserved eucaryotic protein that is required for both SCF (Skp1p/Cdc53p-Cullin-F-box)-mediated ubiquitination and kinetochore function in yeast. We show here that Sgt1p is also involved in the cyclic AMP (cAMP) pathway in Saccharomyces cerevisiae. SGT1 is an allele.specific suppressor of cdc35-1, a thermosensitive mutation in the leucine-rich repeat domain of the adenylyl cyclase Cyr1p/Cdc35p. We demonstrate that Sgt1p and Cyr1p/Cdc35p physically interact and that the activity of the cAMP pathway is affected in an sgt1 conditional mutant. Sequence analysis suggests that Sgt1p has features of a cochaperone. Thus, Sgt1p is a novel activator of adenylyl cyclase in S. cerevisiae and may function in the assembly or the conformational activation of specific multiprotein complexes.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036692080&partnerID=8YFLogxK
U2 - 10.1128/EC.1.4.568-582.2002
DO - 10.1128/EC.1.4.568-582.2002
M3 - Article
C2 - 12456005
AN - SCOPUS:0036692080
VL - 1
SP - 568
EP - 582
JO - Eukaryotic Cell
JF - Eukaryotic Cell
SN - 1535-9778
IS - 4
ER -