Sgt1 dimerization is negatively regulated by protein kinase CK2-mediated phosphorylation at Ser361

Parkil K. Bansal, Ashutosh Mishra, Anthony A. High, Rashid Abdelle, Katsumi Kitagawa

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The kinetochore, which consists of centromere DNA and structural proteins, is essential for proper chromosome segregation in eukaryotes. In budding yeast, Sgt1 and Hsp90 are required for the binding of Skp1 to Ctf13 (a component of the core kinetochore complex CBF3) and therefore for the assembly of CBF3. We have previously shown that Sgt1 dimerization is important for this kinetochore assembly mechanism. In this study, we report that protein kinase CK2 phosphorylates Ser361 on Sgt1, and this phosphorylation inhibits Sgt1 dimerization.

Original languageEnglish (US)
Pages (from-to)18692-18698
Number of pages7
JournalJournal of Biological Chemistry
Volume284
Issue number28
DOIs
StatePublished - Jul 10 2009

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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