Sexual dimorphism in activation of placental autophagy in obese women with evidence for fetal programming from a placenta-specific mouse model

Sribalasubashini Muralimanoharan, Xiaoli Gao, Susan E Weintraub, Leslie Myatt, Alina Maloyan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

ABSTRACT: The incidence of maternal obesity and its co-morbidities (diabetes, cardiovascular disease) continues to increase at an alarming rate, with major public health implications. In utero exposure to maternal obesity has been associated with development of cardiovascular and metabolic diseases in the offspring as a result of developmental programming. The placenta regulates maternal-fetal metabolism and shows significant changes in its function with maternal obesity. Autophagy is a cell-survival process, which is responsible for the degradation of damaged organelles and misfolded proteins. Here we show an activation of autophagosomal formation and autophagosome-lysosome fusion in placentas of males but not females from overweight (OW) and obese (OB) women vs. normal weight (NW) women. However, total autophagic activity in these placentas appeared to be decreased as it showed an increase in SQSTM1/p62 and a decrease in lysosomal biogenesis. A mouse model with a targeted deletion of the essential autophagy gene Atg7 in placental tissue showed significant placental abnormalities comparable to those seen in human placenta with maternal obesity. These included a decrease in expression of mitochondrial genes and antioxidants, and decreased lysosomal biogenesis. Strikingly, the knockout mice were developmentally programmed as they showed an increased sensitivity to high-fat diet-induced obesity, hyperglycemia, hyperinsulinemia, increased adiposity, and cardiac remodeling. In summary, our results indicate a sexual dimorphism in placental autophagy in response to maternal obesity. We also show that autophagy plays an important role in placental function and that inhibition of placental autophagy programs the offspring to obesity, and to metabolic and cardiovascular diseases.

Original languageEnglish (US)
Pages (from-to)752-769
Number of pages18
JournalAutophagy
Volume12
Issue number5
DOIs
StatePublished - May 3 2016

Fingerprint

Autophagy
Fetal Development
Sex Characteristics
Placenta
Obesity
Mothers
Cardiovascular Diseases
Metabolic Diseases
Maternal Exposure
Mitochondrial Genes
Essential Genes
Adiposity
Hyperinsulinism
High Fat Diet
Lysosomes
Knockout Mice
Hyperglycemia
Organelles
Cell Survival
Public Health

Keywords

  • fetal programming
  • high fat diet
  • maternal obesity
  • metabolomics
  • placental function
  • sex differences

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Sexual dimorphism in activation of placental autophagy in obese women with evidence for fetal programming from a placenta-specific mouse model. / Muralimanoharan, Sribalasubashini; Gao, Xiaoli; Weintraub, Susan E; Myatt, Leslie; Maloyan, Alina.

In: Autophagy, Vol. 12, No. 5, 03.05.2016, p. 752-769.

Research output: Contribution to journalArticle

Muralimanoharan, Sribalasubashini ; Gao, Xiaoli ; Weintraub, Susan E ; Myatt, Leslie ; Maloyan, Alina. / Sexual dimorphism in activation of placental autophagy in obese women with evidence for fetal programming from a placenta-specific mouse model. In: Autophagy. 2016 ; Vol. 12, No. 5. pp. 752-769.
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