Abstract
Mice with a termination codon mutation in exon 2 of the cystic fibrosis (CF) gene were generated using homologous recombination in embryonic stem cells. Animals homozygous for the mutant allele display a severe intestinal phenotype similar to that previously reported for CF mutant mice. The null nature of this allele was demonstrated by the absence of detectable wild-type mRNA, by the absence of detectable CFTR in the serous gland collecting ducts of salivary tissues, and by the lack of cAMP-mediated short-circuit current responses in colonic epithelium of mutant animals.
Original language | English (US) |
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Pages (from-to) | 177-187 |
Number of pages | 11 |
Journal | Somatic Cell and Molecular Genetics |
Volume | 21 |
Issue number | 3 |
DOIs | |
State | Published - May 1995 |
ASJC Scopus subject areas
- Genetics
- Cell Biology