TY - JOUR
T1 - Seven genes that are differentially transcribed in colorectal tumor cell lines
AU - Nimmrich, Inko
AU - Erdmann, Silke
AU - Melchers, Ute
AU - Finke, Ulrich
AU - Hentsch, Sebastian
AU - Moyer, Mary Pat
AU - Hoffmann, Ingrid
AU - Müller, Oliver
N1 - Funding Information:
This study was supported by grants from Deutsche Forschungsgemeinschaft (grant MU 1091/8-1), Deutsche Krebshilfe (grant 10-1474-Mü2), and Bundesministerium für Bildung, Forschung und Technologie (grant 0311420).
PY - 2000/11/10
Y1 - 2000/11/10
N2 - To identify genes which are differentially transcribed in colorectal tumor cells, we compared the two human tumor cell lines, SW480 and HCT116, with the cell line, NCM460, from normal colon epithelium as a control. Using the methods of differential display reverse transcription PCR and Northern blot hybridization, we detected the differential transcription of seven genes: cholecystokinin, reticulocalbin, Rab5 guanine nucleotide exchange factor Rabex5, caldesmon, differentiation related gene 1 (drg1), taxol resistant associated gene 3 (Trag-3) and the gene for the placental protein, diff33. The yet unidentified cDNA of the human Rabex5 gene and the 3' untranslated region of the human caldesmon gene were cloned. Copyright (C) 2000 Elsevier Science Ireland Ltd.
AB - To identify genes which are differentially transcribed in colorectal tumor cells, we compared the two human tumor cell lines, SW480 and HCT116, with the cell line, NCM460, from normal colon epithelium as a control. Using the methods of differential display reverse transcription PCR and Northern blot hybridization, we detected the differential transcription of seven genes: cholecystokinin, reticulocalbin, Rab5 guanine nucleotide exchange factor Rabex5, caldesmon, differentiation related gene 1 (drg1), taxol resistant associated gene 3 (Trag-3) and the gene for the placental protein, diff33. The yet unidentified cDNA of the human Rabex5 gene and the 3' untranslated region of the human caldesmon gene were cloned. Copyright (C) 2000 Elsevier Science Ireland Ltd.
KW - Colorectal tumor progression
KW - Differential display reverse transcription polymerase chain reaction
KW - Differential transcription
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U2 - 10.1016/S0304-3835(00)00553-X
DO - 10.1016/S0304-3835(00)00553-X
M3 - Article
C2 - 11098082
AN - SCOPUS:0034634753
SN - 0304-3835
VL - 160
SP - 37
EP - 43
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -