Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

Ande Bao, William T Phillips, Beth Goins, Howard S Mcguff, Xiangpeng Zheng, F. Ross Woolley, Mohan Natarajan, Cristina Santoyo, Frank R Miller, Randal A Otto

Research output: Contribution to journalArticle

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Abstract

Objective: To develop and characterize a new head and neck cancer animal model. Study design: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[18F] fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET) imaging characteristics were studied too. Results: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm3 on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. 18F-FDG PET study has shown that 18F-FDG accumulated in tumors. Conclusions: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.

Original languageEnglish (US)
Pages (from-to)853-857
Number of pages5
JournalOtolaryngology - Head and Neck Surgery
Volume135
Issue number6
DOIs
StatePublished - Dec 2006

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Nude Rats
Heterografts
Neoplasms
Epidermal Growth Factor Receptor
Animal Models
Deoxyglucose
Hematoxylin
Eosine Yellowish-(YS)
Positron-Emission Tomography
Immunohistochemistry
Staining and Labeling
Carcinoma, squamous cell of head and neck
Fluorodeoxyglucose F18
Head and Neck Neoplasms
Pathology
Cell Line

ASJC Scopus subject areas

  • Otorhinolaryngology

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Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats. / Bao, Ande; Phillips, William T; Goins, Beth; Mcguff, Howard S; Zheng, Xiangpeng; Woolley, F. Ross; Natarajan, Mohan; Santoyo, Cristina; Miller, Frank R; Otto, Randal A.

In: Otolaryngology - Head and Neck Surgery, Vol. 135, No. 6, 12.2006, p. 853-857.

Research output: Contribution to journalArticle

Bao, Ande ; Phillips, William T ; Goins, Beth ; Mcguff, Howard S ; Zheng, Xiangpeng ; Woolley, F. Ross ; Natarajan, Mohan ; Santoyo, Cristina ; Miller, Frank R ; Otto, Randal A. / Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats. In: Otolaryngology - Head and Neck Surgery. 2006 ; Vol. 135, No. 6. pp. 853-857.
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AU - Phillips, William T

AU - Goins, Beth

AU - Mcguff, Howard S

AU - Zheng, Xiangpeng

AU - Woolley, F. Ross

AU - Natarajan, Mohan

AU - Santoyo, Cristina

AU - Miller, Frank R

AU - Otto, Randal A

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