Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

Ande Bao; William T. Phillips; Beth Goins; Howard S. McGuff; Xiangpeng Zheng; F. Ross Woolley; Mohan Natarajan; Cristina Santoyo; Frank R. Miller; Randal A. Otto

doi:10.1016/j.otohns.2006.06.1257

Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

Ande Bao, William T. Phillips, Beth Goins, Howard S. McGuff, Xiangpeng Zheng, F. Ross Woolley, Mohan Natarajan, Cristina Santoyo, Frank R. Miller, Randal A. Otto

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Objective: To develop and characterize a new head and neck cancer animal model. Study design: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[¹⁸F] fluoro-2-deoxy-D-glucose (¹⁸F-FDG) positron emission tomography (PET) imaging characteristics were studied too. Results: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm³ on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. ¹⁸F-FDG PET study has shown that ¹⁸F-FDG accumulated in tumors. Conclusions: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.

Original language	English (US)
Pages (from-to)	853-857
Number of pages	5
Journal	Otolaryngology - Head and Neck Surgery
Volume	135
Issue number	6
DOIs	https://doi.org/10.1016/j.otohns.2006.06.1257
State	Published - Dec 2006

ASJC Scopus subject areas

Surgery
Otorhinolaryngology

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10.1016/j.otohns.2006.06.1257

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title = "Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats",

abstract = "Objective: To develop and characterize a new head and neck cancer animal model. Study design: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[18F] fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET) imaging characteristics were studied too. Results: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm3 on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. 18F-FDG PET study has shown that 18F-FDG accumulated in tumors. Conclusions: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.",

author = "Ande Bao and Phillips, {William T.} and Beth Goins and McGuff, {Howard S.} and Xiangpeng Zheng and Woolley, {F. Ross} and Mohan Natarajan and Cristina Santoyo and Miller, {Frank R.} and Otto, {Randal A.}",

year = "2006",

month = dec,

doi = "10.1016/j.otohns.2006.06.1257",

language = "English (US)",

volume = "135",

pages = "853--857",

journal = "Otolaryngology - Head and Neck Surgery",

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T1 - Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

AU - Bao, Ande

AU - Phillips, William T.

AU - Goins, Beth

AU - McGuff, Howard S.

AU - Zheng, Xiangpeng

AU - Woolley, F. Ross

AU - Natarajan, Mohan

AU - Santoyo, Cristina

AU - Miller, Frank R.

AU - Otto, Randal A.

PY - 2006/12

Y1 - 2006/12

N2 - Objective: To develop and characterize a new head and neck cancer animal model. Study design: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[18F] fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET) imaging characteristics were studied too. Results: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm3 on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. 18F-FDG PET study has shown that 18F-FDG accumulated in tumors. Conclusions: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.

AB - Objective: To develop and characterize a new head and neck cancer animal model. Study design: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[18F] fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET) imaging characteristics were studied too. Results: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm3 on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. 18F-FDG PET study has shown that 18F-FDG accumulated in tumors. Conclusions: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.

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Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

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