Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

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27 Scopus citations

Abstract

Objective: To develop and characterize a new head and neck cancer animal model. Study design: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[18F] fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET) imaging characteristics were studied too. Results: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm3 on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. 18F-FDG PET study has shown that 18F-FDG accumulated in tumors. Conclusions: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.

Original languageEnglish (US)
Pages (from-to)853-857
Number of pages5
JournalOtolaryngology - Head and Neck Surgery
Volume135
Issue number6
DOIs
StatePublished - Dec 2006

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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