SETD2 regulates SLC family transporter-mediated sodium and glucose reabsorptions in renal tubule

Taku Mitome; Hiromichi Wakui; Kengo Azushima; Tatsuki Uehara; Ryosuke Jikuya; Shinji Ohtake; Go Noguchi; Sachi Kawaura; Yasuhiro Iribe; Kota Aomori; Tomoyuki Tatenuma; Hiroki Ito; Takashi Kawahara; Mitsuru Komeya; Yusuke Ito; Kentaro Muraoka; Mitsuko Furuya; Ikuma Kato; Satoshi Fujii; Kiyotaka Nagahama; Akira Nishiyama; Tomohiko Tamura; Yayoi Kimura; Tatsukata Kawagoe; Nobuhisa Mizuki; Gang Huang; Hiroji Uemura; Masahiro Yao; Kazuhide Makiyama; Kouichi Tamura; Hisashi Hasumi

doi:10.1016/j.bbrc.2024.150730

SETD2 regulates SLC family transporter-mediated sodium and glucose reabsorptions in renal tubule

Taku Mitome, Hiromichi Wakui, Kengo Azushima, Tatsuki Uehara, Ryosuke Jikuya, Shinji Ohtake, Go Noguchi, Sachi Kawaura, Yasuhiro Iribe, Kota Aomori, Tomoyuki Tatenuma, Hiroki Ito, Takashi Kawahara, Mitsuru Komeya, Yusuke Ito, Kentaro Muraoka, Mitsuko Furuya, Ikuma Kato, Satoshi Fujii, Kiyotaka NagahamaAkira Nishiyama, Tomohiko Tamura, Yayoi Kimura, Tatsukata Kawagoe, Nobuhisa Mizuki, Gang Huang, Hiroji Uemura, Masahiro Yao, Kazuhide Makiyama, Kouichi Tamura, Hisashi Hasumi

Research output: Contribution to journal › Article › peer-review

Abstract

A regulatory mechanism for SLC family transporters, critical transporters for sodium and glucose reabsorptions in renal tubule, is incompletely understood. Here, we report an important regulation of SLC family transporter by SETD2, a chromatin remodeling gene whose alterations have been found in a subset of kidney cancers. Kidney-specific inactivation of Setd2 resulted in hypovolemia with excessive urine excretion in mouse and interestingly, RNA-sequencing analysis of Setd2-deficient murine kidney exhibited decreased expressions of SLC family transporters, critical transporters for sodium and glucose reabsorptions in renal tubule. Importantly, inactivation of Setd2 in murine kidney displayed attenuated dapagliflozin-induced diuresis and glucose excretion, further supporting that SETD2 might regulate SLCfamily transporter-mediated sodium and glucose reabsorptions in renal tubule. These data uncover an important regulation of SLC family transporter by SETD2, which may illuminate a crosstalk between metabolism and epigenome in renal tubule.

Original language	English (US)
Article number	150730
Journal	Biochemical and Biophysical Research Communications
Volume	734
DOIs	https://doi.org/10.1016/j.bbrc.2024.150730
State	Published - Nov 19 2024
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2024.150730

Cite this

Mitome, T., Wakui, H., Azushima, K., Uehara, T., Jikuya, R., Ohtake, S., Noguchi, G., Kawaura, S., Iribe, Y., Aomori, K., Tatenuma, T., Ito, H., Kawahara, T., Komeya, M., Ito, Y., Muraoka, K., Furuya, M., Kato, I., Fujii, S., ... Hasumi, H. (2024). SETD2 regulates SLC family transporter-mediated sodium and glucose reabsorptions in renal tubule. Biochemical and Biophysical Research Communications, 734, Article 150730. https://doi.org/10.1016/j.bbrc.2024.150730

Mitome, T, Wakui, H, Azushima, K, Uehara, T, Jikuya, R, Ohtake, S, Noguchi, G, Kawaura, S, Iribe, Y, Aomori, K, Tatenuma, T, Ito, H, Kawahara, T, Komeya, M, Ito, Y, Muraoka, K, Furuya, M, Kato, I, Fujii, S, Nagahama, K, Nishiyama, A, Tamura, T, Kimura, Y, Kawagoe, T, Mizuki, N, Huang, G, Uemura, H, Yao, M, Makiyama, K, Tamura, K & Hasumi, H 2024, 'SETD2 regulates SLC family transporter-mediated sodium and glucose reabsorptions in renal tubule', Biochemical and Biophysical Research Communications, vol. 734, 150730. https://doi.org/10.1016/j.bbrc.2024.150730

@article{a1168226123c423daf39b7eee6cbe8c7,

title = "SETD2 regulates SLC family transporter-mediated sodium and glucose reabsorptions in renal tubule",

abstract = "A regulatory mechanism for SLC family transporters, critical transporters for sodium and glucose reabsorptions in renal tubule, is incompletely understood. Here, we report an important regulation of SLC family transporter by SETD2, a chromatin remodeling gene whose alterations have been found in a subset of kidney cancers. Kidney-specific inactivation of Setd2 resulted in hypovolemia with excessive urine excretion in mouse and interestingly, RNA-sequencing analysis of Setd2-deficient murine kidney exhibited decreased expressions of SLC family transporters, critical transporters for sodium and glucose reabsorptions in renal tubule. Importantly, inactivation of Setd2 in murine kidney displayed attenuated dapagliflozin-induced diuresis and glucose excretion, further supporting that SETD2 might regulate SLCfamily transporter-mediated sodium and glucose reabsorptions in renal tubule. These data uncover an important regulation of SLC family transporter by SETD2, which may illuminate a crosstalk between metabolism and epigenome in renal tubule.",

author = "Taku Mitome and Hiromichi Wakui and Kengo Azushima and Tatsuki Uehara and Ryosuke Jikuya and Shinji Ohtake and Go Noguchi and Sachi Kawaura and Yasuhiro Iribe and Kota Aomori and Tomoyuki Tatenuma and Hiroki Ito and Takashi Kawahara and Mitsuru Komeya and Yusuke Ito and Kentaro Muraoka and Mitsuko Furuya and Ikuma Kato and Satoshi Fujii and Kiyotaka Nagahama and Akira Nishiyama and Tomohiko Tamura and Yayoi Kimura and Tatsukata Kawagoe and Nobuhisa Mizuki and Gang Huang and Hiroji Uemura and Masahiro Yao and Kazuhide Makiyama and Kouichi Tamura and Hisashi Hasumi",

note = "Publisher Copyright: {\textcopyright} 2024 Elsevier Inc.",

year = "2024",

month = nov,

day = "19",

doi = "10.1016/j.bbrc.2024.150730",

language = "English (US)",

volume = "734",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - SETD2 regulates SLC family transporter-mediated sodium and glucose reabsorptions in renal tubule

AU - Mitome, Taku

AU - Wakui, Hiromichi

AU - Azushima, Kengo

AU - Uehara, Tatsuki

AU - Jikuya, Ryosuke

AU - Ohtake, Shinji

AU - Noguchi, Go

AU - Kawaura, Sachi

AU - Iribe, Yasuhiro

AU - Aomori, Kota

AU - Tatenuma, Tomoyuki

AU - Ito, Hiroki

AU - Kawahara, Takashi

AU - Komeya, Mitsuru

AU - Ito, Yusuke

AU - Muraoka, Kentaro

AU - Furuya, Mitsuko

AU - Kato, Ikuma

AU - Fujii, Satoshi

AU - Nagahama, Kiyotaka

AU - Nishiyama, Akira

AU - Tamura, Tomohiko

AU - Kimura, Yayoi

AU - Kawagoe, Tatsukata

AU - Mizuki, Nobuhisa

AU - Huang, Gang

AU - Uemura, Hiroji

AU - Yao, Masahiro

AU - Makiyama, Kazuhide

AU - Tamura, Kouichi

AU - Hasumi, Hisashi

PY - 2024/11/19

Y1 - 2024/11/19

N2 - A regulatory mechanism for SLC family transporters, critical transporters for sodium and glucose reabsorptions in renal tubule, is incompletely understood. Here, we report an important regulation of SLC family transporter by SETD2, a chromatin remodeling gene whose alterations have been found in a subset of kidney cancers. Kidney-specific inactivation of Setd2 resulted in hypovolemia with excessive urine excretion in mouse and interestingly, RNA-sequencing analysis of Setd2-deficient murine kidney exhibited decreased expressions of SLC family transporters, critical transporters for sodium and glucose reabsorptions in renal tubule. Importantly, inactivation of Setd2 in murine kidney displayed attenuated dapagliflozin-induced diuresis and glucose excretion, further supporting that SETD2 might regulate SLCfamily transporter-mediated sodium and glucose reabsorptions in renal tubule. These data uncover an important regulation of SLC family transporter by SETD2, which may illuminate a crosstalk between metabolism and epigenome in renal tubule.

AB - A regulatory mechanism for SLC family transporters, critical transporters for sodium and glucose reabsorptions in renal tubule, is incompletely understood. Here, we report an important regulation of SLC family transporter by SETD2, a chromatin remodeling gene whose alterations have been found in a subset of kidney cancers. Kidney-specific inactivation of Setd2 resulted in hypovolemia with excessive urine excretion in mouse and interestingly, RNA-sequencing analysis of Setd2-deficient murine kidney exhibited decreased expressions of SLC family transporters, critical transporters for sodium and glucose reabsorptions in renal tubule. Importantly, inactivation of Setd2 in murine kidney displayed attenuated dapagliflozin-induced diuresis and glucose excretion, further supporting that SETD2 might regulate SLCfamily transporter-mediated sodium and glucose reabsorptions in renal tubule. These data uncover an important regulation of SLC family transporter by SETD2, which may illuminate a crosstalk between metabolism and epigenome in renal tubule.

UR - http://www.scopus.com/inward/record.url?scp=85205369697&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85205369697&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2024.150730

DO - 10.1016/j.bbrc.2024.150730

M3 - Article

C2 - 39366177

AN - SCOPUS:85205369697

SN - 0006-291X

VL - 734

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

M1 - 150730

ER -

SETD2 regulates SLC family transporter-mediated sodium and glucose reabsorptions in renal tubule

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this