Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Owen Kavanagh; Mary K. Estes; Amanda Reeck; Ravikiran M. Raju; Antone R. Opekun; Mark A. Gilger; David Y. Graham; Robert L. Atmar

doi:10.1128/CVI.00039-11

Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Owen Kavanagh
, Mary K. Estes
, Amanda Reeck
, Ravikiran M. Raju
, Antone R. Opekun
, Mark A. Gilger
, David Y. Graham
, Robert L. Atmar

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

Original language	English (US)
Pages (from-to)	1187-1190
Number of pages	4
Journal	Clinical and Vaccine Immunology
Volume	18
Issue number	7
DOIs	https://doi.org/10.1128/CVI.00039-11
State	Published - Jul 2011
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)
Clinical Biochemistry
Immunology and Allergy
Immunology

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10.1128/CVI.00039-11

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abstract = "A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).",

author = "Owen Kavanagh and Estes, \{Mary K.\} and Amanda Reeck and Raju, \{Ravikiran M.\} and Opekun, \{Antone R.\} and Gilger, \{Mark A.\} and Graham, \{David Y.\} and Atmar, \{Robert L.\}",

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doi = "10.1128/CVI.00039-11",

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AU - Kavanagh, Owen

AU - Estes, Mary K.

AU - Reeck, Amanda

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AU - Opekun, Antone R.

AU - Gilger, Mark A.

AU - Graham, David Y.

AU - Atmar, Robert L.

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AB - A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

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U2 - 10.1128/CVI.00039-11

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M3 - Article

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AN - SCOPUS:79960143778

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Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Abstract

ASJC Scopus subject areas

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