Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Owen Kavanagh, Mary K. Estes, Amanda Reeck, Ravikiran M. Raju, Antone R. Opekun, Mark A. Gilger, David Y. Graham, Robert L. Atmar

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

Original languageEnglish (US)
Pages (from-to)1187-1190
Number of pages4
JournalClinical and Vaccine Immunology
Volume18
Issue number7
DOIs
StatePublished - Jul 2011
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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