Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Owen Kavanagh; Mary K. Estes; Amanda Reeck; Ravikiran M. Raju; Antone R. Opekun; Mark A. Gilger; David Y. Graham; Robert L. Atmar

doi:10.1128/CVI.00039-11

Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Owen Kavanagh, Mary K. Estes, Amanda Reeck, Ravikiran M. Raju, Antone R. Opekun, Mark A. Gilger, David Y. Graham, Robert L. Atmar

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

Original language	English (US)
Pages (from-to)	1187-1190
Number of pages	4
Journal	Clinical and Vaccine Immunology
Volume	18
Issue number	7
DOIs	https://doi.org/10.1128/CVI.00039-11
State	Published - Jul 2011
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)
Clinical Biochemistry
Immunology and Allergy
Immunology

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10.1128/CVI.00039-11

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abstract = "A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).",

author = "Owen Kavanagh and Estes, {Mary K.} and Amanda Reeck and Raju, {Ravikiran M.} and Opekun, {Antone R.} and Gilger, {Mark A.} and Graham, {David Y.} and Atmar, {Robert L.}",

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AU - Kavanagh, Owen

AU - Estes, Mary K.

AU - Reeck, Amanda

AU - Raju, Ravikiran M.

AU - Opekun, Antone R.

AU - Gilger, Mark A.

AU - Graham, David Y.

AU - Atmar, Robert L.

PY - 2011/7

Y1 - 2011/7

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AB - A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

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Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Abstract

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