Abstract
In order to improve disease-free survival and potentially a cure, it is necessary to identify more potent leukemia antigen. Here, we defined the acute monocytic leukemia-associated antigen (LAA) recognized by the humoral immune system for the first time. We have applied the method of serologic analysis of recombinant cDNA expression library (SEREX) on acute monocytic leukemia (FAB M5), followed by DNA sequencing and analyzing of positive clones. Then, the reactivity of normal and other leukemia sera with positive clones were performed. Thirty-five distinct novel antigens reactive with autologous IgG were identified by SEREX analysis on an acute monocytic leukemia patient and were characterized according to cDNA sequence and the reactivity with allogeneic sera. Twenty of the 35 antigens identified in this study were recognized by IgG antibodies in normal sera, and the remaining 15 were recognized exclusively by sera from allogeneic leukemia patients but not by normal donor sera, suggested that the immune response to these 15 antigens are leukemia related. The 15 immunogenic antigens detected by immune responses in the autologous host facilitate the identification of epitopes recognized by antigen-specific cytotoxic T lymphocytes (CTL) and are potential candidates for diagnosis and immunotherapy in acute myeloid leukemia (AML).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 503-509 |
| Number of pages | 7 |
| Journal | Leukemia Research |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2005 |
| Externally published | Yes |
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Keywords
- Acute leukemia
- Leukemia-associated antigens
- SEREX
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology
Cite this
Serological identification of immunogenic antigens in acute monocytic leukemia. / Chen, Gang; Zhang, Wanggang; Cao, Xingmei; Li, Fuyang; Liu, Xinping; Yao, Libo.
In: Leukemia Research, Vol. 29, No. 5, 05.2005, p. 503-509.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serological identification of immunogenic antigens in acute monocytic leukemia
AU - Chen, Gang
AU - Zhang, Wanggang
AU - Cao, Xingmei
AU - Li, Fuyang
AU - Liu, Xinping
AU - Yao, Libo
PY - 2005/5
Y1 - 2005/5
N2 - In order to improve disease-free survival and potentially a cure, it is necessary to identify more potent leukemia antigen. Here, we defined the acute monocytic leukemia-associated antigen (LAA) recognized by the humoral immune system for the first time. We have applied the method of serologic analysis of recombinant cDNA expression library (SEREX) on acute monocytic leukemia (FAB M5), followed by DNA sequencing and analyzing of positive clones. Then, the reactivity of normal and other leukemia sera with positive clones were performed. Thirty-five distinct novel antigens reactive with autologous IgG were identified by SEREX analysis on an acute monocytic leukemia patient and were characterized according to cDNA sequence and the reactivity with allogeneic sera. Twenty of the 35 antigens identified in this study were recognized by IgG antibodies in normal sera, and the remaining 15 were recognized exclusively by sera from allogeneic leukemia patients but not by normal donor sera, suggested that the immune response to these 15 antigens are leukemia related. The 15 immunogenic antigens detected by immune responses in the autologous host facilitate the identification of epitopes recognized by antigen-specific cytotoxic T lymphocytes (CTL) and are potential candidates for diagnosis and immunotherapy in acute myeloid leukemia (AML).
AB - In order to improve disease-free survival and potentially a cure, it is necessary to identify more potent leukemia antigen. Here, we defined the acute monocytic leukemia-associated antigen (LAA) recognized by the humoral immune system for the first time. We have applied the method of serologic analysis of recombinant cDNA expression library (SEREX) on acute monocytic leukemia (FAB M5), followed by DNA sequencing and analyzing of positive clones. Then, the reactivity of normal and other leukemia sera with positive clones were performed. Thirty-five distinct novel antigens reactive with autologous IgG were identified by SEREX analysis on an acute monocytic leukemia patient and were characterized according to cDNA sequence and the reactivity with allogeneic sera. Twenty of the 35 antigens identified in this study were recognized by IgG antibodies in normal sera, and the remaining 15 were recognized exclusively by sera from allogeneic leukemia patients but not by normal donor sera, suggested that the immune response to these 15 antigens are leukemia related. The 15 immunogenic antigens detected by immune responses in the autologous host facilitate the identification of epitopes recognized by antigen-specific cytotoxic T lymphocytes (CTL) and are potential candidates for diagnosis and immunotherapy in acute myeloid leukemia (AML).
KW - Acute leukemia
KW - Leukemia-associated antigens
KW - SEREX
UR - http://www.scopus.com/inward/record.url?scp=14744277041&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14744277041&partnerID=8YFLogxK
U2 - 10.1016/j.leukres.2004.09.013
DO - 10.1016/j.leukres.2004.09.013
M3 - Article
C2 - 15755502
AN - SCOPUS:14744277041
VL - 29
SP - 503
EP - 509
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 5
ER -