Serological identification of immunogenic antigens in acute monocytic leukemia

Gang Chen, Wanggang Zhang, Xingmei Cao, Fuyang Li, Xinping Liu, Libo Yao

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

In order to improve disease-free survival and potentially a cure, it is necessary to identify more potent leukemia antigen. Here, we defined the acute monocytic leukemia-associated antigen (LAA) recognized by the humoral immune system for the first time. We have applied the method of serologic analysis of recombinant cDNA expression library (SEREX) on acute monocytic leukemia (FAB M5), followed by DNA sequencing and analyzing of positive clones. Then, the reactivity of normal and other leukemia sera with positive clones were performed. Thirty-five distinct novel antigens reactive with autologous IgG were identified by SEREX analysis on an acute monocytic leukemia patient and were characterized according to cDNA sequence and the reactivity with allogeneic sera. Twenty of the 35 antigens identified in this study were recognized by IgG antibodies in normal sera, and the remaining 15 were recognized exclusively by sera from allogeneic leukemia patients but not by normal donor sera, suggested that the immune response to these 15 antigens are leukemia related. The 15 immunogenic antigens detected by immune responses in the autologous host facilitate the identification of epitopes recognized by antigen-specific cytotoxic T lymphocytes (CTL) and are potential candidates for diagnosis and immunotherapy in acute myeloid leukemia (AML).

Original languageEnglish (US)
Pages (from-to)503-509
Number of pages7
JournalLeukemia Research
Volume29
Issue number5
DOIs
StatePublished - May 2005

Keywords

  • Acute leukemia
  • Leukemia-associated antigens
  • SEREX

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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