Serologic discrimination of human T cell lymphotropic virus infection by using a synthetic peptide-based enzyme immunoassay

R. B. Lal, D. L. Rudolph, M. D. Lairmore, R. F. Khabbaz, M. Garfield, J. E. Coligan, T. M. Folks

    Research output: Contribution to journalArticle

    38 Scopus citations

    Abstract

    Synthetic peptides corresponding with unique regions of the envelope glycoproteins (gp46) of human T cell lymphotropic viruses (HTLVs) were used in an enzyme immunoassay to determine if HTLV-I and -II infections could be discriminated. Two synthetic HTLV-I sequence-derived peptides, Env-1 (amino acids 191-215) and Env-5 (amino acids 242-257), reacted with 92% and 100% of the serum specimens (n = 52) from HTLV-I-infected persons, respectively. Although a small percentage (8.6%) of serum specimens from persons infected with HTLV-II cross-reacted with Env-1, none of these specimens reacted with Env-5. Peptide Env-2 encoded by the envelope region of HTLV-II (amino acids 187-210) reacted with serum specimens from both HTLV-I (94%)- and HTLV-II (74%)-infected patients, whereas Env-6, another HTLV-II peptide (amino acids 238-254), reacted with <6% of the specimens. Therefore, the Env-5 peptide with amino acid sequence SerProAsnValSerValProSerSerSerSerThrProLeuLeuTyr represents an immunodominant domain of HTLV-I that is recognized by serum antibodies from all HTLV-I-infected persons. Moreover, the Env-5-based ELISA allows a categorical distinction between the closely related HTLV-I and -II infections.

    Original languageEnglish (US)
    Pages (from-to)41-46
    Number of pages6
    JournalJournal of Infectious Diseases
    Volume163
    Issue number1
    DOIs
    StatePublished - Jan 1 1991

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Infectious Diseases

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  • Cite this

    Lal, R. B., Rudolph, D. L., Lairmore, M. D., Khabbaz, R. F., Garfield, M., Coligan, J. E., & Folks, T. M. (1991). Serologic discrimination of human T cell lymphotropic virus infection by using a synthetic peptide-based enzyme immunoassay. Journal of Infectious Diseases, 163(1), 41-46. https://doi.org/10.1093/infdis/163.1.41