Sera from preeclamptic patients contain factor(s) that stimulate prostacyclin production by human endothelial cells

D. W. Branch, D. J. Dudley, S. LaMarche, M. D. Mitchell

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

A relative decrease in endothelial cell prostacyclin production may be pivotal in the genesis of preeclampsia. We determined the effect of sera from preeclamptic women on prostacyclin production by monolayers of normal term human umbilical vein endothelial cells. Endothelial cells were incubated with media containing serum from patients with preeclampsia, non-hypertensive, gestational age-matched pregnant controls, or normal non-pregnant controls (N = 7, all groups). 6-Keto-prostaglandin F, the stable metabolite of prostacyclin, was measured directly in the culture medium by radioimmunoassay. Treatment with preeclamptic sera, when associated with a statistically significant increase in prostacyclin metabolite production by endothelial cells. Thus, sera from women with preeclampsia stimulate rather than inhibit prostacyclin production by endothelial cells. We speculate that there is a factor in the sera of women with preeclampsia that functions to activate endothelial cells or which may play a role in the homeostatic mechanisms to balance reduced prostacyclin output in preeclampsia.

Original languageEnglish (US)
Pages (from-to)191-195
Number of pages5
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume45
Issue number3
DOIs
StatePublished - Mar 1992
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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