Sequence and organization of the human N-formyl peptide receptor-encoding gene

Philip M. Murphy, H. Lee Tiffany, David McDermott, Sunil K. Ahuja

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The human FPR1 gene encodes the N-formyl peptide receptor, a G protein-coupled receptor (GPCR) that mediates the activation of mature myeloid cells by bacterial N-formyl oligopeptides. To investigate the molecular basis for myeloid-specific production of this receptor, we have cloned and sequenced FPRL The gene is organized into three exons and two introns that span 6 kb. The coding block lacks introns. Yet, the transcription start point (tsp) is separated from the start codon by 4902 bp consisting of three exons and two large introns. Two mRNAs are produced by alternative splicing of exon 2 in HL-60 neutrophils and normal blood monocytes. The region 5′ to the tsp contains three pyrimidine-rich segments, a feature that has been observed in other myeloid-specific genes. One complete Alu repeat is found in each intron and in the 3′-flanking region 317 bp downstream of the polyadenylation signal. Thus, FPR1 is a small myeloid-specific gene that is expressed as two alternatively spliced mRNAs encoding the same protein.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalGene
Volume133
Issue number2
DOIs
StatePublished - Nov 15 1993

Keywords

  • Alu repeat
  • G protein-coupled receptor
  • chemo-attractant receptor
  • intronless coding region
  • myeloid-specific gene

ASJC Scopus subject areas

  • Genetics

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