Separation of epidermal cells by density centrifugation: a new technique for studies on normal and pathological differentiation

Susan M. Fischer, Karen D.G. Nelson, John J. Reiners, Aurora Viaji, Jill C. Pelling, Thomas J. Slaga

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Murine keratinocytes, isolated by flotation trypsinization of skin, can be separated into five groups by centrifugation through Percoll, a colloidal silica gradient. Within each group a good correlation was found between density, plating efficiency, morphological appearance, DNA synthesis, and degree of keratinization/cornification. This method can be applied equally well to fetal, newborn, or adult keratinocytes and should be useful in a variety of studies including isolation of subpopulations of pathological cell types, work on chalones and hyperplastic diseases such as psoriasis, and in vitro transformation studies.

Original languageEnglish (US)
Pages (from-to)43-49
Number of pages7
JournalJournal of Cutaneous Pathology
Volume9
Issue number1
DOIs
StatePublished - Feb 1982
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Dermatology

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