Separation and quantification of choline and acetylcholine by thermospray liquid chromatography/mass spectrometry

Daniel J. Liberato, Alfred L. Yergey, Susan T. Weintraub

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The development of sensitive analytical techniques for use in the identification and quantification of molecules of biological origin remains a constant challenge. Often the compounds of interest are present in a complex mixture and require extensive sample preparation prior to analysis. Thermospray liquid chromatography/mass spectrometry (LC/MS) has been shown to be a method which allows for both separation of target molecules from complex mixtures and sensitive specific detection using a mass spectrometer. In this work thermospray LC/MS is used for the direct detection of intact, underivatized choline and acetylcholine from mouse brain homogenate. The results of our analysis corroborate previous analyses using a variety of indirect measurement techniques and thereby show that this simple direct analysis has wide potential applicability.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalBiological Mass Spectrometry
Volume13
Issue number4
DOIs
StatePublished - Apr 1986

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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