Separation and quantification of choline and acetylcholine by thermospray liquid chromatography/mass spectrometry.

D. J. Liberato, A. L. Yergey, Susan E Weintraub

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The development of sensitive analytical techniques for use in the identification and qualification of molecules of biological origin remains a constant challenge. Often the compounds of interest are present in a complex mixture and require extensive sample preparation prior to analysis. Thermospray liquid chromatography/mass spectrometry (LC/MS) has been shown to be a method which allows for both separation of target molecules from complex mixtures and sensitive specific detection using a mass spectrometer. In this work thermospray LC/MS is used for the direct detection of intact, underivatized choline and acetylcholine from mouse brain homogenate. The results of our analysis corroborate previous analyses using a variety of indirect measurement techniques and thereby show that this simple direct analysis has wide potential applicability.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalBiomedical & environmental mass spectrometry
Volume13
Issue number4
StatePublished - Apr 1986

Fingerprint

Liquid chromatography
Choline
Complex Mixtures
Liquid Chromatography
Acetylcholine
Mass spectrometry
Mass Spectrometry
Molecules
Mass spectrometers
Brain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pollution

Cite this

Separation and quantification of choline and acetylcholine by thermospray liquid chromatography/mass spectrometry. / Liberato, D. J.; Yergey, A. L.; Weintraub, Susan E.

In: Biomedical & environmental mass spectrometry, Vol. 13, No. 4, 04.1986, p. 171-174.

Research output: Contribution to journalArticle

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