Separate mechanisms activate σ(B) of Bacillus subtilis in response to environmental and metabolic stresses

U. Voelker, A. Voelker, B. Maul, M. Hecker, A. Dufour, W. G. Haldenwang

Research output: Contribution to journalArticlepeer-review

186 Scopus citations

Abstract

σ(B) is a secondary sigma factor that controls the general stress response of Bacillus subtilis. σ(B)-dependent transcription is induced by the activation of σ(B) itself, a process that involves release of σ(B) from an inhibitory complex with its primary regulator, RsbW. σ(B) becomes available to RNA polymerase when RsbW forms a complex with an additional regulatory protein (RsbV) and, because of this, fails to bind σ(B). Using Western blot (immunoblot) analyses, reporter gene fusion assays, and measurements of nucleotide pool sizes, we provide evidence for two independent processes that promote the binding of RsbW to RsbV. The first occurs during carbon limitation or entry into stationary phase. Activation of σ(B) under these circumstances correlates with a drop in the intracellular levels of ATP and may be a direct consequence of ATP levels on RsbW's binding preference. The second activation process relies on the product of a third regulatory gene, rsbU. RsbU is dispensable for σ(B) activation during carbon limitation or stationary phase but is needed for activation of σ(B) in response to any of a number of different environmental insults (ethanol treatment, salt or acid shock, etc.). RsbU, or a process dependent on it, alters RsbW binding without regard for intracellular levels of ATP. In at least some instances, the effects of multiple inducing stimuli are additive. The data are consistent with RsbW being a regulator at which distinct signals from separate effectors can be integrated to modulate σ(B) activity.

Original languageEnglish (US)
Pages (from-to)3771-3780
Number of pages10
JournalJournal of bacteriology
Volume177
Issue number13
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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