Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva

Y. W.Francis Lam, Daniel C.N. Chan, Sandra Y. Rodriguez, John H. Lintakoon, Thu Hong Lam

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 μl) was extracted into methylene chloride. Chromatographic separation was achieved with a C18 column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 μg/ml (r2 ≥ 0.997). Intra-day and inter-day coefficients of variation were <5.5 and ≤9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.

Original languageEnglish (US)
Pages (from-to)166-171
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume612
Issue number1
DOIs
StatePublished - Jan 29 1993

ASJC Scopus subject areas

  • Chemistry(all)

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