Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva

Y. W.Francis Lam; Daniel C.N. Chan; Sandra Y. Rodriguez; John H. Lintakoon; Thu Hong Lam

doi:10.1016/0378-4347(93)80381-D

Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva

Y. W.Francis Lam, Daniel C.N. Chan, Sandra Y. Rodriguez, John H. Lintakoon, Thu Hong Lam

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 μl) was extracted into methylene chloride. Chromatographic separation was achieved with a C₁₈ column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 μg/ml (r² ≥ 0.997). Intra-day and inter-day coefficients of variation were <5.5 and ≤9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.

Original language	English (US)
Pages (from-to)	166-171
Number of pages	6
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	612
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(93)80381-D
State	Published - Jan 29 1993

ASJC Scopus subject areas

General Chemistry

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title = "Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva",

abstract = "A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 μl) was extracted into methylene chloride. Chromatographic separation was achieved with a C18 column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 μg/ml (r2 ≥ 0.997). Intra-day and inter-day coefficients of variation were <5.5 and ≤9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.",

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AU - Chan, Daniel C.N.

AU - Rodriguez, Sandra Y.

AU - Lintakoon, John H.

AU - Lam, Thu Hong

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AB - A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 μl) was extracted into methylene chloride. Chromatographic separation was achieved with a C18 column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 μg/ml (r2 ≥ 0.997). Intra-day and inter-day coefficients of variation were <5.5 and ≤9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.

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Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva

Abstract

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