Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Arne Hansen; Yidong Chen; Jason M. Inman; Quang N. Phan; Zhi Qing Qi; Charlie C. Xiang; Miklos Palkovits; Natasha Cherman; Sergei A. Kuznetsov; Pamela G. Robey; Eva Mezey; Michael J. Brownstein

doi:10.1038/nmeth977

Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Arne Hansen
, Yidong Chen
, Jason M. Inman
, Quang N. Phan
, Zhi Qing Qi
, Charlie C. Xiang
, Miklos Palkovits
, Natasha Cherman
, Sergei A. Kuznetsov
, Pamela G. Robey
, Eva Mezey
, Michael J. Brownstein

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

Original language	English (US)
Pages (from-to)	35-37
Number of pages	3
Journal	Nature Methods
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/nmeth977
State	Published - Jan 2007
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth977

Cite this

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title = "Sensitive and specific method for detecting G protein-coupled receptor mRNAs",

abstract = "G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.",

author = "Arne Hansen and Yidong Chen and Inman, \{Jason M.\} and Phan, \{Quang N.\} and Qi, \{Zhi Qing\} and Xiang, \{Charlie C.\} and Miklos Palkovits and Natasha Cherman and Kuznetsov, \{Sergei A.\} and Robey, \{Pamela G.\} and Eva Mezey and Brownstein, \{Michael J.\}",

year = "2007",

month = jan,

doi = "10.1038/nmeth977",

language = "English (US)",

volume = "4",

pages = "35--37",

journal = "Nature Methods",

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T1 - Sensitive and specific method for detecting G protein-coupled receptor mRNAs

AU - Hansen, Arne

AU - Chen, Yidong

AU - Inman, Jason M.

AU - Phan, Quang N.

AU - Qi, Zhi Qing

AU - Xiang, Charlie C.

AU - Palkovits, Miklos

AU - Cherman, Natasha

AU - Kuznetsov, Sergei A.

AU - Robey, Pamela G.

AU - Mezey, Eva

AU - Brownstein, Michael J.

PY - 2007/1

Y1 - 2007/1

N2 - G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

AB - G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

UR - https://www.scopus.com/pages/publications/33845927817

UR - https://www.scopus.com/pages/publications/33845927817#tab=citedBy

U2 - 10.1038/nmeth977

DO - 10.1038/nmeth977

M3 - Article

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AN - SCOPUS:33845927817

SN - 1548-7091

VL - 4

SP - 35

EP - 37

JO - Nature Methods

JF - Nature Methods

IS - 1

ER -

Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Abstract

ASJC Scopus subject areas

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