Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Arne Hansen, Yidong Chen, Jason M. Inman, Quang N. Phan, Zhi Qing Qi, Charlie C. Xiang, Miklos Palkovits, Natasha Cherman, Sergei A. Kuznetsov, Pamela G. Robey, Eva Mezey, Michael J. Brownstein

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

Original languageEnglish (US)
Pages (from-to)35-37
Number of pages3
JournalNature Methods
Volume4
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

Fingerprint

G-Protein-Coupled Receptors
Messenger RNA
Multiplex Polymerase Chain Reaction
Microarrays
Mesenchymal Stromal Cells
Gene expression
Assays
Bone
Cells
RNA
Gene Expression
Cell Line
Molecules

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Hansen, A., Chen, Y., Inman, J. M., Phan, Q. N., Qi, Z. Q., Xiang, C. C., ... Brownstein, M. J. (2007). Sensitive and specific method for detecting G protein-coupled receptor mRNAs. Nature Methods, 4(1), 35-37. https://doi.org/10.1038/nmeth977

Sensitive and specific method for detecting G protein-coupled receptor mRNAs. / Hansen, Arne; Chen, Yidong; Inman, Jason M.; Phan, Quang N.; Qi, Zhi Qing; Xiang, Charlie C.; Palkovits, Miklos; Cherman, Natasha; Kuznetsov, Sergei A.; Robey, Pamela G.; Mezey, Eva; Brownstein, Michael J.

In: Nature Methods, Vol. 4, No. 1, 01.2007, p. 35-37.

Research output: Contribution to journalArticle

Hansen, A, Chen, Y, Inman, JM, Phan, QN, Qi, ZQ, Xiang, CC, Palkovits, M, Cherman, N, Kuznetsov, SA, Robey, PG, Mezey, E & Brownstein, MJ 2007, 'Sensitive and specific method for detecting G protein-coupled receptor mRNAs', Nature Methods, vol. 4, no. 1, pp. 35-37. https://doi.org/10.1038/nmeth977
Hansen, Arne ; Chen, Yidong ; Inman, Jason M. ; Phan, Quang N. ; Qi, Zhi Qing ; Xiang, Charlie C. ; Palkovits, Miklos ; Cherman, Natasha ; Kuznetsov, Sergei A. ; Robey, Pamela G. ; Mezey, Eva ; Brownstein, Michael J. / Sensitive and specific method for detecting G protein-coupled receptor mRNAs. In: Nature Methods. 2007 ; Vol. 4, No. 1. pp. 35-37.
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