Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Arne Hansen; Yidong Chen; Jason M. Inman; Quang N. Phan; Zhi Qing Qi; Charlie C. Xiang; Miklos Palkovits; Natasha Cherman; Sergei A. Kuznetsov; Pamela G. Robey; Eva Mezey; Michael J. Brownstein

doi:10.1038/nmeth977

Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Arne Hansen, Yidong Chen, Jason M. Inman, Quang N. Phan, Zhi Qing Qi, Charlie C. Xiang, Miklos Palkovits, Natasha Cherman, Sergei A. Kuznetsov, Pamela G. Robey, Eva Mezey, Michael J. Brownstein

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

Original language	English (US)
Pages (from-to)	35-37
Number of pages	3
Journal	Nature Methods
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/nmeth977
State	Published - Jan 2007
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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title = "Sensitive and specific method for detecting G protein-coupled receptor mRNAs",

abstract = "G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.",

author = "Arne Hansen and Yidong Chen and Inman, {Jason M.} and Phan, {Quang N.} and Qi, {Zhi Qing} and Xiang, {Charlie C.} and Miklos Palkovits and Natasha Cherman and Kuznetsov, {Sergei A.} and Robey, {Pamela G.} and Eva Mezey and Brownstein, {Michael J.}",

year = "2007",

month = jan,

doi = "10.1038/nmeth977",

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journal = "Nature Methods",

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AU - Hansen, Arne

AU - Chen, Yidong

AU - Inman, Jason M.

AU - Phan, Quang N.

AU - Qi, Zhi Qing

AU - Xiang, Charlie C.

AU - Palkovits, Miklos

AU - Cherman, Natasha

AU - Kuznetsov, Sergei A.

AU - Robey, Pamela G.

AU - Mezey, Eva

AU - Brownstein, Michael J.

PY - 2007/1

Y1 - 2007/1

N2 - G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

AB - G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

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JO - Nature Methods

JF - Nature Methods

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ER -

Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Abstract

ASJC Scopus subject areas

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