Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins

Ellen R. Goldman, Igor L. Medintz, Andrew Hayhurst, George P. Anderson, J. Matthew Mauro, Brent L. Iverson, George Georgiou, Hedi Mattoussi

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    81 Scopus citations

    Abstract

    We report a simple and versatile approach for the conjugation of luminescent CdSe-ZnS core-shell quantum dots (QDs) to proteins through coordination of engineered C-terminal oligohistidine sequences. Several histidine tail containing proteins were self-assembled onto the QD surface using this method. A recombinant antibody specific for the high explosive 2,4,6-trinitrotoluene (TNT) was conjugated to QDs through a carboxy terminal histidine tail and the bioconjugate used to detect TNT by competitive immunoassay. TNT was detected over the range of 10 μg/ml down to 41 ng/ml using the scFv conjugated to QDs. These results open up the possibility to conjugate luminescent QDs to a whole range of proteins to form QD bioconjugates that can be effectively used in bio-oriented applications, such as sensing, imaging, immunoassay and other diagnostics.

    Original languageEnglish (US)
    Pages (from-to)63-67
    Number of pages5
    JournalAnalytica Chimica Acta
    Volume534
    Issue number1
    DOIs
    StatePublished - Apr 4 2005

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    Keywords

    • Affinity tag
    • Bioconjugation
    • Histidine tag
    • Immunoassay
    • Quantum dot
    • Single chain antibody
    • TNT
    • scFv

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Environmental Chemistry
    • Spectroscopy

    Cite this

    Goldman, E. R., Medintz, I. L., Hayhurst, A., Anderson, G. P., Mauro, J. M., Iverson, B. L., Georgiou, G., & Mattoussi, H. (2005). Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins. Analytica Chimica Acta, 534(1), 63-67. https://doi.org/10.1016/j.aca.2004.03.079