Selective modulation of the functions of a conserved DNA motor by a histone fold complex

Xiaoyu Xue; Koyi Choi; Jaclyn N. Bonner; Barnabas Szakal; Yu Hung Chen; Alma Papusha; Dorina Saro; Hengyao Niu; Grzegorz Ira; Dana Branzei; Patrick Sung; Xiaolan Zhao

doi:10.1101/gad.259143.115

Selective modulation of the functions of a conserved DNA motor by a histone fold complex

Xiaoyu Xue, Koyi Choi, Jaclyn N. Bonner, Barnabas Szakal, Yu Hung Chen, Alma Papusha, Dorina Saro, Hengyao Niu, Grzegorz Ira, Dana Branzei, Patrick Sung, Xiaolan Zhao

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes. Here, we show that the conserved histone fold MHF complex promotes Mph1mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replicationassociated repair through binding to Mph1 but not DNA. Thus, scMHF is a function-specific enhancer of Mph1 that enables flexible response to different genome repair situations.

Original language	English (US)
Pages (from-to)	1000-1005
Number of pages	6
Journal	Genes and Development
Volume	29
Issue number	10
DOIs	https://doi.org/10.1101/gad.259143.115
State	Published - 2015
Externally published	Yes

Keywords

DNA motor proteins
Genome replication
Histone fold proteins
Homologous recombination
Replication fork repair
Structural maintenance of chromosome proteins

ASJC Scopus subject areas

General Medicine

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10.1101/gad.259143.115

Cite this

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title = "Selective modulation of the functions of a conserved DNA motor by a histone fold complex",

abstract = "Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes. Here, we show that the conserved histone fold MHF complex promotes Mph1mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replicationassociated repair through binding to Mph1 but not DNA. Thus, scMHF is a function-specific enhancer of Mph1 that enables flexible response to different genome repair situations.",

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T1 - Selective modulation of the functions of a conserved DNA motor by a histone fold complex

AU - Xue, Xiaoyu

AU - Choi, Koyi

AU - Bonner, Jaclyn N.

AU - Szakal, Barnabas

AU - Chen, Yu Hung

AU - Papusha, Alma

AU - Saro, Dorina

AU - Niu, Hengyao

AU - Ira, Grzegorz

AU - Branzei, Dana

AU - Sung, Patrick

AU - Zhao, Xiaolan

PY - 2015

Y1 - 2015

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AB - Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes. Here, we show that the conserved histone fold MHF complex promotes Mph1mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replicationassociated repair through binding to Mph1 but not DNA. Thus, scMHF is a function-specific enhancer of Mph1 that enables flexible response to different genome repair situations.

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KW - Homologous recombination

KW - Replication fork repair

KW - Structural maintenance of chromosome proteins

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Selective modulation of the functions of a conserved DNA motor by a histone fold complex

Abstract

Keywords

ASJC Scopus subject areas

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