Selective modulation of the functions of a conserved DNA motor by a histone fold complex

Xiaoyu Xue, Koyi Choi, Jaclyn N. Bonner, Barnabas Szakal, Yu Hung Chen, Alma Papusha, Dorina Saro, Hengyao Niu, Grzegorz Ira, Dana Branzei, Patrick Sung, Xiaolan Zhao

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes. Here, we show that the conserved histone fold MHF complex promotes Mph1mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replicationassociated repair through binding to Mph1 but not DNA. Thus, scMHF is a function-specific enhancer of Mph1 that enables flexible response to different genome repair situations.

Original languageEnglish (US)
Pages (from-to)1000-1005
Number of pages6
JournalGenes and Development
Volume29
Issue number10
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • DNA motor proteins
  • Genome replication
  • Histone fold proteins
  • Homologous recombination
  • Replication fork repair
  • Structural maintenance of chromosome proteins

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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