Selective modification of nuclear proteins by polycyclic aromatic hydrocarbons and by benzo[a]pyrene diol epoxides

Arend Kootstra, Michael C. Macleod, Radhakrishnan Iyer, James K. Selkirk, Thomas J Slaga

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Metabolites of benzo[a]pyrene (B[a]P) have been shown to modify chromosomal proteins with great specificity. Using the (+) and (-) enantiomers of anti-B[a]P diol epoxide to label isolated nuclei we found a remarkable difference in the capacity of these two compounds to modify histones H2A and H3. The (+) enantiomer modified histones H2A and H3, while the (-) enantiomer, which was shown to modify mainly histone H2A, had a much lower affinity for histone H3. We have also examined the selective modification of chromosomal proteins by different polycyclic aromatic hydrocarbons and it was observed that 7,12-dimethylbenz[a]-anthracene (DMBA), 3-methylcholanthrene (3-MC) and B[a]P showed qualitative similarities in terms of their protein binding. This suggests that stereospecific interactions leading to binding of reactive metabolites of DMBA, B[a]P and 3-MC to chromosomal proteins share common features.

Original languageEnglish (US)
Pages (from-to)821-824
Number of pages4
JournalCarcinogenesis
Volume3
Issue number7
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Benzo(a)pyrene
Polycyclic Aromatic Hydrocarbons
Epoxy Compounds
Pyrene
Hydrocarbons
Polycyclic aromatic hydrocarbons
Nuclear Proteins
Histones
Enantiomers
Protein
9,10-Dimethyl-1,2-benzanthracene
Methylcholanthrene
Metabolites
Proteins
Anthracene
Protein Binding
Nucleus
Specificity
Affine transformation
Labels

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Selective modification of nuclear proteins by polycyclic aromatic hydrocarbons and by benzo[a]pyrene diol epoxides. / Kootstra, Arend; Macleod, Michael C.; Iyer, Radhakrishnan; Selkirk, James K.; Slaga, Thomas J.

In: Carcinogenesis, Vol. 3, No. 7, 1982, p. 821-824.

Research output: Contribution to journalArticle

Kootstra, Arend ; Macleod, Michael C. ; Iyer, Radhakrishnan ; Selkirk, James K. ; Slaga, Thomas J. / Selective modification of nuclear proteins by polycyclic aromatic hydrocarbons and by benzo[a]pyrene diol epoxides. In: Carcinogenesis. 1982 ; Vol. 3, No. 7. pp. 821-824.
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