TY - JOUR
T1 - Selective keratinocyte stimulation is sufficient to evoke nociception in mice
AU - Pang, Zixuan
AU - Sakamoto, Takashi
AU - Tiwari, Vinod
AU - Kim, Yu Shin
AU - Yang, Fei
AU - Dong, Xinzhong
AU - Güler, Ali D.
AU - Guan, Yun
AU - Caterina, Michael J.
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 2015/4/1
Y1 - 2015/4/1
N2 - The skin epidermis is densely innervated by peripheral sensory nerve endings. Nociceptive neurons, whose terminals are in close contact with epidermal keratinocytes, can be activated directly by noxious physical and chemical stimuli to trigger pain. However, whether keratinocytes can signal acutely to sensory nerve terminals to initiate pain in vivo remains unclear. Here, using the keratin 5 promoter to selectively express the capsaicin receptor TRPV1 in keratinocytes of TRPV1-knockout mice, we achieved specific stimulation of keratinocytes with capsaicin. Using this approach, we found that keratinocyte stimulation was sufficient to induce strong expression of the neuronal activation marker, c-fos, in laminae I and II of the ipsilateral spinal cord dorsal horn and to evoke acute paw-licking nocifensive behavior and conditioned place aversion. These data provide direct evidence that keratinocyte stimulation is sufficient to evoke acute nociception-related responses.
AB - The skin epidermis is densely innervated by peripheral sensory nerve endings. Nociceptive neurons, whose terminals are in close contact with epidermal keratinocytes, can be activated directly by noxious physical and chemical stimuli to trigger pain. However, whether keratinocytes can signal acutely to sensory nerve terminals to initiate pain in vivo remains unclear. Here, using the keratin 5 promoter to selectively express the capsaicin receptor TRPV1 in keratinocytes of TRPV1-knockout mice, we achieved specific stimulation of keratinocytes with capsaicin. Using this approach, we found that keratinocyte stimulation was sufficient to induce strong expression of the neuronal activation marker, c-fos, in laminae I and II of the ipsilateral spinal cord dorsal horn and to evoke acute paw-licking nocifensive behavior and conditioned place aversion. These data provide direct evidence that keratinocyte stimulation is sufficient to evoke acute nociception-related responses.
UR - http://www.scopus.com/inward/record.url?scp=84943812640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84943812640&partnerID=8YFLogxK
U2 - 10.1097/j.pain.0000000000000092
DO - 10.1097/j.pain.0000000000000092
M3 - Article
C2 - 25790456
AN - SCOPUS:84943812640
VL - 156
SP - 656
EP - 665
JO - Pain
JF - Pain
SN - 0304-3959
IS - 4
ER -