Selective isolation and culture of a proliferating epithelial cell population from the hamster trachea

William E. Goldman, Joel B. Baseman

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

A reliable cell isolation technique was developed to allow the cultivation of cells from the hamster respiratory tract. Repeated thermolysin treatments and gradient centrifugation yielded a cell culture completely free from contamination by fibroblasts. Viable cells could be isolated from as little tissue as a single hamster trachea, but in vitro proliferation occurred only if the hamster was less than 4 months of age. The cultured cells could be repeatedly passaged and subcultured for weeks by employing normal tissue culture techniques. Morphologically, the monolayers appeared to be a homogeneous population of epithelial cells, and successful cloning of freshly isolated single cells resulted in apparently identical cultures. The epithelial origin of these cells was also suggested by continued growth in minimum essential medium with d-valine substituted for l-valine. The relative ease with which this cell type can be isolated, cultured, and manipulated in vitro should encourage its application as a model of the respiratory epithelium.

Original languageEnglish (US)
Pages (from-to)313-319
Number of pages7
JournalIn Vitro
Volume16
Issue number4
DOIs
StatePublished - Apr 1 1980
Externally publishedYes

Keywords

  • cell culture
  • epithelial cells
  • model system
  • respiratory tract
  • trachea

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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