Selective in vitro inhibition of an antibody response to purified acetylcholine receptor by using anti-idiotypic antibodies coupled to the A chain of ricin

R. M. DeShambo, K. A. Krolick

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

In vitro antibody responses by rat lymph node cells against purified acetylcholine receptor (AChR) were shown to be inhibitable by protein conjugates prepared with anti-idiotypic antibody and the toxic A chain of ricin. The idiotype specificity of the cytotoxicity was demonstrated by the inability of the same immunotoxin to inhibit an unrelated antibody response (anti-KLH) and by abrogation of specific toxicity in the presence of unconjugated anti-idiotype or antigen (AChR). Furthermore, immunotoxin prepared with an irrelevant antibody specificity failed to significantly inhibit either the anti-AChR or control antibody responses. Therefore, we suggest that idiotype-specific immunotoxins may be a useful addition or alternative to presently employed immunotherapies for autoimmune disease.

Original languageEnglish (US)
Pages (from-to)3135-3139
Number of pages5
JournalJournal of Immunology
Volume137
Issue number10
StatePublished - 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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