Selection of strains for quality assessment of the disk induction method for detection of inducible clindamycin resistance in staphylococci: A CLSI collaborative study

Adrian M. Zelazny, Mary Jane Ferraro, Anita Glennen, Janet F. Hindler, Linda M. Mann, Susan Munro, Patrick R. Murray, L. Barth Reller, Fred C. Tenover, James H. Jorgensen

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

A nine-laboratory collaborative study was conducted to select positive and negative quality assessment control strains for the detection of inducible clindamycin resistance in staphylococci. Four strains of Staphylococcus aureus were tested as unknowns on 10 different days in each laboratory using the recently recommended CLSI (formerly NCCLS) disk diffusion method and the inoculum purity control method. Strains contained either macrolide-lincosamide- streptogramin B (MLSB) resistance genes encoded by erm(A) or erm(C) or a macrolide resistance efflux pump encoded by msr(A). Based upon the results of this study, strain UT 32 (now designated ATCC strain BAA-977) containing erm(A) is recommended as the positive control organism for inducible clindamycin resistance. Strain UT 25 (now designated ATCC BAA-976), which harbors the efflux pump encoded by msr(A), is recommended as the negative control organism.

Original languageEnglish (US)
Pages (from-to)2613-2615
Number of pages3
JournalJournal of clinical microbiology
Volume43
Issue number6
DOIs
StatePublished - Jun 2005

ASJC Scopus subject areas

  • Microbiology (medical)

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