1. 1. The Chironomus thummi thummi hemoglobins have been isolated and separated into monomeric and dimeric Hbs by gel chromatography and into single components using disc electrophoresis. 2. 2. Essential properties of CTT Hbs were checked (molecular wt, u.v. and visible absorption spectra, component composition). 3. 3. Seasonal variability of Hb content and component composition was found. Concentration of total Hb in hemolymph was 2.7-3.0% and 4.5-4.9% in spring and summer generation respectively, dimeric to monomeric Hbs ratio was in these two generations 1.27-1.54 and 2.13-2.37. 4. 4. This variability was discussed in evolutional and physiological terms and interpreted as adaptations to variations in ecological conditions (particularly to oxygen tension and temperature).
|Original language||English (US)|
|Number of pages||4|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|State||Published - 1985|
ASJC Scopus subject areas
- Molecular Biology