Seasonal variability of hemoglobin content and component composition of Chironomus thummi thummi larvae

Wanda Leyko, Pawel Osmulski

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

1. 1. The Chironomus thummi thummi hemoglobins have been isolated and separated into monomeric and dimeric Hbs by gel chromatography and into single components using disc electrophoresis. 2. 2. Essential properties of CTT Hbs were checked (molecular wt, u.v. and visible absorption spectra, component composition). 3. 3. Seasonal variability of Hb content and component composition was found. Concentration of total Hb in hemolymph was 2.7-3.0% and 4.5-4.9% in spring and summer generation respectively, dimeric to monomeric Hbs ratio was in these two generations 1.27-1.54 and 2.13-2.37. 4. 4. This variability was discussed in evolutional and physiological terms and interpreted as adaptations to variations in ecological conditions (particularly to oxygen tension and temperature).

Original languageEnglish (US)
Pages (from-to)613-616
Number of pages4
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume80
Issue number3
DOIs
StatePublished - 1985
Externally publishedYes

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Chironomidae
Disc Electrophoresis
Hemolymph
Gel Chromatography
Larva
Hemoglobins
Oxygen
Temperature
Chromatography
Electrophoresis
Chemical analysis
Absorption spectra
Gels

Cite this

Seasonal variability of hemoglobin content and component composition of Chironomus thummi thummi larvae. / Leyko, Wanda; Osmulski, Pawel.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 80, No. 3, 1985, p. 613-616.

Research output: Contribution to journalArticle

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