Schistosoma mansoni: The developmental regulation and immunolocalization of antioxidant enzymes

Haiping Mei, Philip T. LoVerde

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

Antioxidant enzymes from S. mansoni, cytosolic Cu-Zn superoxide dismutase (CT-SOD), signal-peptide-containing SOD (SP-SOD), glutathione peroxidase (GPX), and glutathione transferase (GST) were compared for their relative levels of transcript expression throughout development in a semiquantitative reverse transcriptase-polymerase chain reaction assay. All of the antioxidant enzymes exhibited a similar pattern of developmental regulation. Adult worms have the highest level of specific mRNA compared with larval stages. GST shows the highest level of expression, being approximately 10-fold more abundant than CT-SOD and SP-SOD and 100-fold more abundant than GPX. This order of expression was nearly consistent for all the developmental stages studied. To localize the antioxidant enzymes, immunofluorescence staining was performed on 3-hr schistosomula and adult worms. GPX, SP-SOD, and CT-SOD were all found to be associated with the adult tegument and gut epithelium, SP-SOD was also associated with organelle and cell membranes of parenchymal cells and interestingly with the spines of adult worms. Schistosomula, on the other hand, showed little immunofluorescence. These studies further demonstrate the developmental regulation of antioxidant enzymes and localize them to the host-parasite interface, supporting the notion that they have a role in allowing adult worms to evade immune attack.

Original languageEnglish (US)
Pages (from-to)69-78
Number of pages10
JournalExperimental Parasitology
Volume86
Issue number1
DOIs
StatePublished - May 1997

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Keywords

  • Antioxidant enzymes
  • CT-SOD, cytosolic Cu-Zn superoxide dismutase
  • GPX, glutathione peroxidase
  • GST, glutathione S- transferase
  • RT- PCR, reverse transcriptase-polymerase chain reaction
  • SP SOD, signal-peptide-containing Cu-Zn SOD
  • Schistosoma mansoni
  • cDNA, complementary deoxyribonucleic acid
  • confocal microscopy
  • mRNA, messenger ribonucleic acid

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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