TY - JOUR
T1 - Schistosoma mansoni p48 eggshell protein gene
T2 - characterization, developmentally regulated expression and comparison to the p14 eggshell protein gene
AU - Chen, Li ly
AU - Rekosh, David M.
AU - LoVerde, Philip T.
N1 - Funding Information:
We thank Dr. John Cordingley for cDNA clone F4 and Ms. Theresa Wnuk for outstanding secretarial service. This research was supported by NIAID grant AI127219. This work was conducted as part of the Center for
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/5
Y1 - 1992/5
N2 - Egg production by worm pairs is a major cause of pathogenesis in schistosomiasis. To further the understanding of female reproductive development, we have isolated and characterized a complete copy of an eggshell protein precursor gene, p48. Sequence analysis reveals that the gene has 3 open reading frames and does not contain an intron. One of the open reading frames, ORF1, encodes a polypeptide of 50 kDa which shows strong homology to insect chorion proteins. Determination of the position of the mRNA cap-site facilitated identification of putative regulatory elements in the 5′ upstream region of the gene. Some of these elements (e.g. TCACGT) have been shown to play a role in the regulation of chorion gene expression in insects. p48 mRNA is detectable only in mature female worms and the ability to detect the mRNA coincides temporally with worm pairing. Quantitative comparisons, during female reproductive development, of p48 transcripts to those from another eggshell protein precursor gene, p14, show that the p48 mRNA is significantly less abundant than p14 mRNA. In mature female worms, p48 mRNA can only be detected in vitelline cells. Antibodies made against the polypeptide sequence deduced from ORF1 of the p48 gene recognize a 50-kDa molecule in extracts from mature female worms, but not in extracts from immature females or males.
AB - Egg production by worm pairs is a major cause of pathogenesis in schistosomiasis. To further the understanding of female reproductive development, we have isolated and characterized a complete copy of an eggshell protein precursor gene, p48. Sequence analysis reveals that the gene has 3 open reading frames and does not contain an intron. One of the open reading frames, ORF1, encodes a polypeptide of 50 kDa which shows strong homology to insect chorion proteins. Determination of the position of the mRNA cap-site facilitated identification of putative regulatory elements in the 5′ upstream region of the gene. Some of these elements (e.g. TCACGT) have been shown to play a role in the regulation of chorion gene expression in insects. p48 mRNA is detectable only in mature female worms and the ability to detect the mRNA coincides temporally with worm pairing. Quantitative comparisons, during female reproductive development, of p48 transcripts to those from another eggshell protein precursor gene, p14, show that the p48 mRNA is significantly less abundant than p14 mRNA. In mature female worms, p48 mRNA can only be detected in vitelline cells. Antibodies made against the polypeptide sequence deduced from ORF1 of the p48 gene recognize a 50-kDa molecule in extracts from mature female worms, but not in extracts from immature females or males.
KW - Chorion gene
KW - Developmentally regulated gene expression
KW - Eggshell protein gene
KW - Schistosoma mansoni
KW - Schistosome female reproductive development
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U2 - 10.1016/0166-6851(92)90034-H
DO - 10.1016/0166-6851(92)90034-H
M3 - Article
C2 - 1625706
AN - SCOPUS:0026582301
SN - 0166-6851
VL - 52
SP - 39
EP - 52
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -