Schistosoma mansoni p48 eggshell protein gene: characterization, developmentally regulated expression and comparison to the p14 eggshell protein gene

Li ly Chen, David M. Rekosh, Philip T. LoVerde

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Egg production by worm pairs is a major cause of pathogenesis in schistosomiasis. To further the understanding of female reproductive development, we have isolated and characterized a complete copy of an eggshell protein precursor gene, p48. Sequence analysis reveals that the gene has 3 open reading frames and does not contain an intron. One of the open reading frames, ORF1, encodes a polypeptide of 50 kDa which shows strong homology to insect chorion proteins. Determination of the position of the mRNA cap-site facilitated identification of putative regulatory elements in the 5′ upstream region of the gene. Some of these elements (e.g. TCACGT) have been shown to play a role in the regulation of chorion gene expression in insects. p48 mRNA is detectable only in mature female worms and the ability to detect the mRNA coincides temporally with worm pairing. Quantitative comparisons, during female reproductive development, of p48 transcripts to those from another eggshell protein precursor gene, p14, show that the p48 mRNA is significantly less abundant than p14 mRNA. In mature female worms, p48 mRNA can only be detected in vitelline cells. Antibodies made against the polypeptide sequence deduced from ORF1 of the p48 gene recognize a 50-kDa molecule in extracts from mature female worms, but not in extracts from immature females or males.

Original languageEnglish (US)
Pages (from-to)39-52
Number of pages14
JournalMolecular and Biochemical Parasitology
Volume52
Issue number1
DOIs
StatePublished - May 1992

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Keywords

  • Chorion gene
  • Developmentally regulated gene expression
  • Eggshell protein gene
  • Schistosoma mansoni
  • Schistosome female reproductive development

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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