Schistosoma mansoni: Identification and characterization of schistosomula polypeptides

Shohreh Miller, David M. Rekosh, Philip T. LoVerde

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Schistosomula proteins separated by a two-dimensional (NEPHGE) gel system identify 94 major silver-stained polypeptides. When compared to polypeptides similarly separated from cercariae and adult worms; cercariae share the same polypeptides as schistosomula, adult worms share ca. 60% of the polypeptides. A group of five schistosomula polypeptides 15-31 kDa (apparent pI 8.2-8.9) was not found in adult worm extracts. To identify which polypeptides were immunogens, Western blots of the NEPHGE gels were probed with sera either from humans with chronic schistosomiasis or from mice vaccinated with irradiated cercariae. For characterization studies, polyclonal antibodies were made against the five schistosomula-specific and selected immunogenic polypeptides by immunizing mice with silver-stained spots removed from NEPHGE gels. We show that the polyclonal serum against a polypeptide of 12.5 kDa and an apparent pI of 6.70 mediated complement and eosinophil-dependent killing of schistosomula in an in vitro assay. Epitopes recognized by antibody against the 12.5-kDa polypeptide show a diffuse distribution and are found on flame cells of the excretory system of the schistosomula.

Original languageEnglish (US)
Pages (from-to)249-262
Number of pages14
JournalExperimental Parasitology
Volume69
Issue number2
DOIs
StatePublished - Aug 1989

Keywords

  • (NEPHGE)
  • Flame cells
  • Schistosoma mansoni
  • Two-dimensional gel

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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