Schistosoma mansoni: Identification and analysis of an mRNA and a gene encoding superoxide dismutase ( Cu Zn)

Maryanne C. Simurda; Harry van Keulen; David M. Rekosh; Philip T. LoVerde

doi:10.1016/0014-4894(88)90010-0

Schistosoma mansoni: Identification and analysis of an mRNA and a gene encoding superoxide dismutase ( Cu Zn)

Maryanne C. Simurda, Harry van Keulen, David M. Rekosh, Philip T. LoVerde

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

The sequence of an mRNA encoding superoxide dismutase (EC 1.15.1.1) of Schistosoma mansoni has been determined. The deduced amino acid sequence which it encodes shows 40 to 45% homology with the amino acid sequences of the cytosolic superoxide dismutase from 10 other species, as well as similar homology with the human extracellular form. Hybridization selection of the mRNA by cDNA clone pSM38 and in vitro translation of this mRNA have consistently produced a protein of 20,000 Da and occasionally, a 40,000-Da protein. Both are immunoprecipitable with sera pooled from patients with chronic schistosomiasis. The gene from which this mRNA is transcribed has also been isolated and characterized. It spans 5.1 kb of chromosomal DNA and possesses three exons and two introns which interrupt the coding region. These introns contain the splice junction sequences which fit the consensus sequences observed in mammalian genes. The upstream sequence of the gene shows a region for a potential 'TATA' box and the downstream sequence contains two polyadenylation sites.

Original language	English (US)
Pages (from-to)	73-84
Number of pages	12
Journal	Experimental Parasitology
Volume	67
Issue number	1
DOIs	https://doi.org/10.1016/0014-4894(88)90010-0
State	Published - Oct 1988
Externally published	Yes

Keywords

Schistosoma mansoni
Trematode

ASJC Scopus subject areas

Infectious Diseases
Parasitology
Immunology

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10.1016/0014-4894(88)90010-0

Cite this

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title = "Schistosoma mansoni: Identification and analysis of an mRNA and a gene encoding superoxide dismutase ( Cu Zn)",

abstract = "The sequence of an mRNA encoding superoxide dismutase (EC 1.15.1.1) of Schistosoma mansoni has been determined. The deduced amino acid sequence which it encodes shows 40 to 45% homology with the amino acid sequences of the cytosolic superoxide dismutase from 10 other species, as well as similar homology with the human extracellular form. Hybridization selection of the mRNA by cDNA clone pSM38 and in vitro translation of this mRNA have consistently produced a protein of 20,000 Da and occasionally, a 40,000-Da protein. Both are immunoprecipitable with sera pooled from patients with chronic schistosomiasis. The gene from which this mRNA is transcribed has also been isolated and characterized. It spans 5.1 kb of chromosomal DNA and possesses three exons and two introns which interrupt the coding region. These introns contain the splice junction sequences which fit the consensus sequences observed in mammalian genes. The upstream sequence of the gene shows a region for a potential 'TATA' box and the downstream sequence contains two polyadenylation sites.",

keywords = "Schistosoma mansoni, Trematode",

author = "Simurda, {Maryanne C.} and {van Keulen}, Harry and Rekosh, {David M.} and LoVerde, {Philip T.}",

note = "Funding Information: ACKNOWLEDGMENTS The chronic human sera were a gift from Dr. G. I. Higashi, University of Michigan. We thank Theresa Wnuk for excellent secretarial assistance. This work was funded by grants from NIAID (R22 AI-18867) and the Edna McConnell Clark Foundation. D.M.R. is the recipient of a RCDA (CA-000905) from NCI. This work was conducted as part of the Center for Applied Molecular Biology and Immunology supported by the State University of New York at Buffalo.",

year = "1988",

month = oct,

doi = "10.1016/0014-4894(88)90010-0",

language = "English (US)",

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AU - Rekosh, David M.

AU - LoVerde, Philip T.

N1 - Funding Information: ACKNOWLEDGMENTS The chronic human sera were a gift from Dr. G. I. Higashi, University of Michigan. We thank Theresa Wnuk for excellent secretarial assistance. This work was funded by grants from NIAID (R22 AI-18867) and the Edna McConnell Clark Foundation. D.M.R. is the recipient of a RCDA (CA-000905) from NCI. This work was conducted as part of the Center for Applied Molecular Biology and Immunology supported by the State University of New York at Buffalo.

PY - 1988/10

Y1 - 1988/10

N2 - The sequence of an mRNA encoding superoxide dismutase (EC 1.15.1.1) of Schistosoma mansoni has been determined. The deduced amino acid sequence which it encodes shows 40 to 45% homology with the amino acid sequences of the cytosolic superoxide dismutase from 10 other species, as well as similar homology with the human extracellular form. Hybridization selection of the mRNA by cDNA clone pSM38 and in vitro translation of this mRNA have consistently produced a protein of 20,000 Da and occasionally, a 40,000-Da protein. Both are immunoprecipitable with sera pooled from patients with chronic schistosomiasis. The gene from which this mRNA is transcribed has also been isolated and characterized. It spans 5.1 kb of chromosomal DNA and possesses three exons and two introns which interrupt the coding region. These introns contain the splice junction sequences which fit the consensus sequences observed in mammalian genes. The upstream sequence of the gene shows a region for a potential 'TATA' box and the downstream sequence contains two polyadenylation sites.

AB - The sequence of an mRNA encoding superoxide dismutase (EC 1.15.1.1) of Schistosoma mansoni has been determined. The deduced amino acid sequence which it encodes shows 40 to 45% homology with the amino acid sequences of the cytosolic superoxide dismutase from 10 other species, as well as similar homology with the human extracellular form. Hybridization selection of the mRNA by cDNA clone pSM38 and in vitro translation of this mRNA have consistently produced a protein of 20,000 Da and occasionally, a 40,000-Da protein. Both are immunoprecipitable with sera pooled from patients with chronic schistosomiasis. The gene from which this mRNA is transcribed has also been isolated and characterized. It spans 5.1 kb of chromosomal DNA and possesses three exons and two introns which interrupt the coding region. These introns contain the splice junction sequences which fit the consensus sequences observed in mammalian genes. The upstream sequence of the gene shows a region for a potential 'TATA' box and the downstream sequence contains two polyadenylation sites.

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Schistosoma mansoni: Identification and analysis of an mRNA and a gene encoding superoxide dismutase ( Cu Zn)

Abstract

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