Schistosoma mansoni genome project: An update

Philip T. LoVerde, Hirohisa Hirai, Joseph M. Merrick, Norman H. Lee, Najib El-Sayed

Research output: Contribution to journalReview articlepeer-review

48 Scopus citations

Abstract

A schistosome genome project was initiated by the World Health Organization in 1994 with the notion that the best prospects for identifying new targets for drugs, vaccines, and diagnostic development lie in schistosome gene discovery, development of chromosome maps, whole genome sequencing and genome analysis. Schistosoma mansoni has a haploid genome of 270 Mb contained on 8 pairs of chromosomes. It is estimated that the S. mansoni genome contains between 15000 and 25000 genes. There are approximately 16689 ESTs obtained from diverse libraries representing different developmental stages of S. mansoni, deposited in the NCBI EST database. More than half of the deposited sequences correspond to genes of unknown function. Approximately 40-50% of the sequences form unique clusters, suggesting that approximately 20-25% of the total schistosome genes have been discovered. Efforts to develop low resolution chromosome maps are in progress. There is a genome sequencing program underway that will provide 3X sequence coverage of the S. mansoni genome that will result in approximately 95% gene discovery. The genomics era has provided the resources to usher in the era of functional genomics that will involve microarrays to focus on specific metabolic pathways, proteomics to identify relevant proteins and protein-protein interactions to understand critical parasite pathways. Functional genomics is expected to accelerate the development of control and treatment strategies for schistosomiasis.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalParasitology International
Volume53
Issue number2
DOIs
StatePublished - Jun 1 2004

Keywords

  • BAC, bacterial artificial chromosome
  • CDS, coding sequences
  • Chromosome mapping
  • EST, expressed sequence tag
  • ET, expressed transcript
  • GO, gene ontology
  • Gene discovery
  • Genomics
  • RT-PCR, reverse transcriptase-polymerase chain reaction
  • Schistosoma mansoni

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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