TY - JOUR
T1 - Schistosoma mansoni
T2 - Electrophoretic characterization of strains selected for different levels of infectivity to snails
AU - Fletcher, Madeleine
AU - LoVerde, Philip T.
AU - Richards, Charles S.
N1 - Funding Information:
We thank Drs. V. Ferris, R. M. Cable, D. L. Hartl, and Mr. M. Goldman for critical review of the manuscript; John DeWald and Paul Shade for technical assistance; Dr. Ernest0 Ruiz for providing some of the Puerto Rican isolates of Schistosoma mansoni for this study; and Betty Glick for typing the manuscript. This research was supported, in part, by grants from the Edna McConnell Clark Foundation, from the U.S. National Science Foundation (RIAS SER 7706731), and from the U.S. Office Naval Research (Contract Nl. N00014.78.C.0081).
PY - 1981/12
Y1 - 1981/12
N2 - Individual adult Schistosoma mansoni from strains selected for high or low infectivity to specific strains of the snail intermediate host, Biomphalaria glabrata, were subjected to enzyme electrophoresis on starch gels. Fourteen enzyme systems were analyzed in an attempt to find electrophoretic markers associated with genes for infectivity to snails. The S. mansoni strains were selected from different isolates from Puerto Rico in several strains of B. glabrata. Of an estimated 18 loci, 3 were polymorphic and the remainder monomorphic. For 1 of the 3 polymorphic enzyme loci, lactate dehydrogenase (Ldh, EC 1.1.1.27), phenotype frequencies were correlated with infectivity to snails. In schistosome strains of low infectivity, frequencies of the Ldh-N phenotype ranged between 0.56 and 0.69, while in strains of high infectivity, Ldh-N frequencies were typically 0.91 to 1.00. Whether the correlation is accidental or due to some form of association, such as chromosomal linkage, between the locus responsible for variation in lactate dehydrogenase and a gene for infectivity to snails remains to be determined.
AB - Individual adult Schistosoma mansoni from strains selected for high or low infectivity to specific strains of the snail intermediate host, Biomphalaria glabrata, were subjected to enzyme electrophoresis on starch gels. Fourteen enzyme systems were analyzed in an attempt to find electrophoretic markers associated with genes for infectivity to snails. The S. mansoni strains were selected from different isolates from Puerto Rico in several strains of B. glabrata. Of an estimated 18 loci, 3 were polymorphic and the remainder monomorphic. For 1 of the 3 polymorphic enzyme loci, lactate dehydrogenase (Ldh, EC 1.1.1.27), phenotype frequencies were correlated with infectivity to snails. In schistosome strains of low infectivity, frequencies of the Ldh-N phenotype ranged between 0.56 and 0.69, while in strains of high infectivity, Ldh-N frequencies were typically 0.91 to 1.00. Whether the correlation is accidental or due to some form of association, such as chromosomal linkage, between the locus responsible for variation in lactate dehydrogenase and a gene for infectivity to snails remains to be determined.
KW - Biomphalaria glabrata
KW - Blood fluke
KW - Chromosomal linkage
KW - Differentiation, intraspecific
KW - Enzyme electrophoresis
KW - Genetics
KW - Infectivity
KW - Lactate dehydrogenase (EC 1.1.1.27)
KW - Schistosoma mansoni
KW - Trematode
KW - snail
UR - http://www.scopus.com/inward/record.url?scp=0019742851&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019742851&partnerID=8YFLogxK
U2 - 10.1016/0014-4894(81)90094-1
DO - 10.1016/0014-4894(81)90094-1
M3 - Article
C2 - 7318984
AN - SCOPUS:0019742851
SN - 0014-4894
VL - 52
SP - 362
EP - 370
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3
ER -