TY - JOUR
T1 - Satellite cell functional alterations following cutaneous burn in rats include an increase in their osteogenic potential
AU - Wu, Xiaowu
AU - Rathbone, Christopher R.
N1 - Funding Information:
The authors thank Ms. Melissa Sanchez for expert technical assistance. Research was supported by US Army Medical Research and Materiel Command. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the US Department of Defense or the US Government. The authors are employees of the US Government. This work was prepared as part of his official duties and, as such, there is no copyright to be transferred.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/10
Y1 - 2013/10
N2 - Background: Significant consequences of severe burn include skeletal muscle atrophy and heterotopic ossification (HO). The cellular mechanisms underlying either of these conditions are not known. Whether the functionality of satellite cells stem cells resident in skeletal muscle is affected by changes in circulatory factors following burn was determined to better understand their role in atrophy and HO. Materials and methods: Serum (20%) from sham-treated animals or burned animals (40% total body surface area full-thickness burn) was used to culture satellite cells isolated from either sham or burn animals. Satellite cells were separated based on fiber type (i.e., fast-twitch or slow-twitch in some cases). To gain greater insight into the potential role for satellite cells in controlling muscle mass following burn, the effect of serum taken from burn animals on satellite cell proliferation, migration, and myogenic differentiation was evaluated. Osteogenic differentiation was assessed to evaluate the potential of satellite cells to contribute to HO. Results: Burn serum (BS) increased the proliferative capacity of cells from fast-twitch muscle, and the migratory capacity of satellite cells taken from both fast- and slow-twitch muscles. BS increased both the myogenic and osteogenic differentiation of satellite cells taken from both sham and burn animals. Conclusions: The unexpected increase in myogenic functionality of satellite cells with BS is difficult to rectify, given the degree of atrophy that occurs. However, the increased osteogenic capacity of satellite cells with BS suggests they may play a role in burn-induced HO.
AB - Background: Significant consequences of severe burn include skeletal muscle atrophy and heterotopic ossification (HO). The cellular mechanisms underlying either of these conditions are not known. Whether the functionality of satellite cells stem cells resident in skeletal muscle is affected by changes in circulatory factors following burn was determined to better understand their role in atrophy and HO. Materials and methods: Serum (20%) from sham-treated animals or burned animals (40% total body surface area full-thickness burn) was used to culture satellite cells isolated from either sham or burn animals. Satellite cells were separated based on fiber type (i.e., fast-twitch or slow-twitch in some cases). To gain greater insight into the potential role for satellite cells in controlling muscle mass following burn, the effect of serum taken from burn animals on satellite cell proliferation, migration, and myogenic differentiation was evaluated. Osteogenic differentiation was assessed to evaluate the potential of satellite cells to contribute to HO. Results: Burn serum (BS) increased the proliferative capacity of cells from fast-twitch muscle, and the migratory capacity of satellite cells taken from both fast- and slow-twitch muscles. BS increased both the myogenic and osteogenic differentiation of satellite cells taken from both sham and burn animals. Conclusions: The unexpected increase in myogenic functionality of satellite cells with BS is difficult to rectify, given the degree of atrophy that occurs. However, the increased osteogenic capacity of satellite cells with BS suggests they may play a role in burn-induced HO.
KW - Atrophy
KW - Heterotopic ossification
KW - Muscle precursor cell
KW - Skeletal muscle
KW - Thermal injury
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U2 - 10.1016/j.jss.2013.03.046
DO - 10.1016/j.jss.2013.03.046
M3 - Article
C2 - 23582758
AN - SCOPUS:84883854109
VL - 184
SP - E9-E16
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 2
ER -