Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

Xing Chen; Da Fu Cui; Chang Chun Liu; Hui Li; Wei Xing Zhao

Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

Xing Chen, Da Fu Cui, Chang Chun Liu, Hui Li, Wei Xing Zhao

Research output: Contribution to journal › Article › peer-review

Abstract

A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction (SPE) and the MEMS technology. Oxidized porous silicon with a large surface area as the solid phase matrix for absorption DNA from biologic sample can greatly improve the DNA yield. The factors which can affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by PCR, which can achieve a high efficiency of the amplification.

Original language	English (US)
Pages (from-to)	618-621
Number of pages	4
Journal	Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
Volume	27
Issue number	4
State	Published - Apr 2006
Externally published	Yes

Keywords

MEMS
Oxidized porous silicon
Sample pretreatment microfluidic chip

ASJC Scopus subject areas

General Chemistry

Cite this

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title = "Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood",

abstract = "A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction (SPE) and the MEMS technology. Oxidized porous silicon with a large surface area as the solid phase matrix for absorption DNA from biologic sample can greatly improve the DNA yield. The factors which can affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by PCR, which can achieve a high efficiency of the amplification.",

keywords = "MEMS, Oxidized porous silicon, Sample pretreatment microfluidic chip",

author = "Xing Chen and Cui, {Da Fu} and Liu, {Chang Chun} and Hui Li and Zhao, {Wei Xing}",

year = "2006",

month = apr,

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journal = "Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities",

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TY - JOUR

T1 - Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

AU - Chen, Xing

AU - Cui, Da Fu

AU - Liu, Chang Chun

AU - Li, Hui

AU - Zhao, Wei Xing

PY - 2006/4

Y1 - 2006/4

N2 - A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction (SPE) and the MEMS technology. Oxidized porous silicon with a large surface area as the solid phase matrix for absorption DNA from biologic sample can greatly improve the DNA yield. The factors which can affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by PCR, which can achieve a high efficiency of the amplification.

AB - A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction (SPE) and the MEMS technology. Oxidized porous silicon with a large surface area as the solid phase matrix for absorption DNA from biologic sample can greatly improve the DNA yield. The factors which can affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by PCR, which can achieve a high efficiency of the amplification.

KW - MEMS

KW - Oxidized porous silicon

KW - Sample pretreatment microfluidic chip

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JF - Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities

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Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

Abstract

Keywords

ASJC Scopus subject areas

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