Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

Xing Chen, Da Fu Cui, Chang Chun Liu, Hui Li, Wei Xing Zhao

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction (SPE) and the MEMS technology. Oxidized porous silicon with a large surface area as the solid phase matrix for absorption DNA from biologic sample can greatly improve the DNA yield. The factors which can affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by PCR, which can achieve a high efficiency of the amplification.

Original languageEnglish (US)
Pages (from-to)618-621
Number of pages4
JournalGaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
Volume27
Issue number4
StatePublished - Apr 2006

Keywords

  • MEMS
  • Oxidized porous silicon
  • Sample pretreatment microfluidic chip

ASJC Scopus subject areas

  • Chemistry(all)

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