Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

Xing Chen; Dafu Cui; Changchun Liu; Hui Li; Weixing Zhao

doi:10.1007/s11458-007-0015-7

Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

Xing Chen, Dafu Cui, Changchun Liu, Hui Li, Weixing Zhao

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction and micro electro mechanical system technology. Oxidized porous silicon with the large surface area as the solid phase matrix for absorption of DNA from a biological sample can greatly improve the DNA yield. The factors that could affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by polymerase chain reaction, which can achieve a high efficiency of the amplification.

Original language	English (US)
Pages (from-to)	74-78
Number of pages	5
Journal	Frontiers of Chemistry in China
Volume	2
Issue number	1
DOIs	https://doi.org/10.1007/s11458-007-0015-7
State	Published - Mar 2007
Externally published	Yes

Keywords

Micro electro mechanical system
Oxidized porous silicon
Sample pretreatment microfluific chip

ASJC Scopus subject areas

Chemistry(all)

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10.1007/s11458-007-0015-7

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title = "Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood",

abstract = "A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction and micro electro mechanical system technology. Oxidized porous silicon with the large surface area as the solid phase matrix for absorption of DNA from a biological sample can greatly improve the DNA yield. The factors that could affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by polymerase chain reaction, which can achieve a high efficiency of the amplification.",

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T1 - Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

AU - Chen, Xing

AU - Cui, Dafu

AU - Liu, Changchun

AU - Li, Hui

AU - Zhao, Weixing

PY - 2007/3

Y1 - 2007/3

N2 - A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction and micro electro mechanical system technology. Oxidized porous silicon with the large surface area as the solid phase matrix for absorption of DNA from a biological sample can greatly improve the DNA yield. The factors that could affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by polymerase chain reaction, which can achieve a high efficiency of the amplification.

AB - A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction and micro electro mechanical system technology. Oxidized porous silicon with the large surface area as the solid phase matrix for absorption of DNA from a biological sample can greatly improve the DNA yield. The factors that could affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by polymerase chain reaction, which can achieve a high efficiency of the amplification.

KW - Micro electro mechanical system

KW - Oxidized porous silicon

KW - Sample pretreatment microfluific chip

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Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood

Abstract

Keywords

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