Salts promote activation of fat cell adenylate cyclase by GTP: Special role for sodium ion

M. S. Katz, J. S. Partilla, M. A. Pineyro, C. R. Schneyer, R. I. Gregerman

Research output: Contribution to journalArticle

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Abstract

The effects of GTP on adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] of human and rat fat cell membranes ('ghosts' and purified membranes) were examined in the absence and presence of added inorganic salts. With human ghosts GTP alone (0.1 mM) inhibited enzyme activity by 40% at 30°C and had no significant effect at 37°C. At both temperatures Na+ salts of Cl-, N3 -, and SO4 2- stimulated activity (up to 4-fold basal activity for 200 mM NaN3), with maximal effects at salt concentrations of 100-200 mM. Over the same concentration range these salts also allowed temperature-dependent stimulation by GTP. GTP increased the maximal activity produced by salt alone by about 2-fold at 30°C and about 4-fold at 37°C. Na+ (added as Cl-) was much more effective than other alkali metal cations in promoting activation by GTP. Na+ salts allowed activation of the human enzyme by the GTP analog 5'-guanylyl imidodiphosphate and also promoted stimulation of rat fat cell adenylate cyclase by both nucleotides. In time course studies of human and rat fat cell ghosts, GTP appeared to sustain an initial high rate of salt-stimulated activity, which in the absence of nucleotide subsequently fell to a lower rate, suggesting that salts might activate adenylate cyclase by promoting the stimulatory effect of endogenous membrane-bound GTP. However, with purified human fat cell membranes and a GTP-free system, salts were still stimulatory and promoted activation by added GTP. These results differ from those of previous reports in other systems in which Na+ has promoted only inhibitory effects in GTP regulation of adenylate cyclase.

Original languageEnglish (US)
Pages (from-to)7417-7421
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number12 II
StatePublished - 1981

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Guanosine Triphosphate
Adenylyl Cyclases
Adipocytes
Salts
Sodium
Ions
Nucleotides
Cell Membrane
Guanylyl Imidodiphosphate
Alkali Metals
Sodium Azide
Temperature
Enzyme Activation
Membranes
Cations

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Katz, M. S., Partilla, J. S., Pineyro, M. A., Schneyer, C. R., & Gregerman, R. I. (1981). Salts promote activation of fat cell adenylate cyclase by GTP: Special role for sodium ion. Proceedings of the National Academy of Sciences of the United States of America, 78(12 II), 7417-7421.

Salts promote activation of fat cell adenylate cyclase by GTP : Special role for sodium ion. / Katz, M. S.; Partilla, J. S.; Pineyro, M. A.; Schneyer, C. R.; Gregerman, R. I.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, No. 12 II, 1981, p. 7417-7421.

Research output: Contribution to journalArticle

Katz, M. S. ; Partilla, J. S. ; Pineyro, M. A. ; Schneyer, C. R. ; Gregerman, R. I. / Salts promote activation of fat cell adenylate cyclase by GTP : Special role for sodium ion. In: Proceedings of the National Academy of Sciences of the United States of America. 1981 ; Vol. 78, No. 12 II. pp. 7417-7421.
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