Salt-supplemented medium for testing methicillin-resistant staphylococci with newer β-lactams

J. H. Jorgensen, J. S. Redding, L. A. Maher, P. E. Ramirez

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The addition of 2% NaCl to cation-supplemented Mueller-Hinton broth (CSMHB) was evaluated for microdilution testing of the susceptibility of staphylococci to five cephalosporins, imipenem, amoxicillin-clavulanate, and ticarcillin-clavulanate. With Staphylococcus aureus, NaCl improved the recognition of methicillin (oxacillin) resistance and resistance to cephalothin, cefazolin, cefonicid, and cefotaxime, but did not clearly define resistance to cefamandole, imipenem, or ticarcillin-clavulanate. Resistance to amoxicillin-clavulanate was readily determined, irrespective of the presence of added salt. The addition of 2% NaCl to CSMHB did not significantly improve detection of resistance to any of the beta-lactams among coagulase-negative staphylococci, the routine addition of 2% NaCl to oxacillin or methicillin tests with staphylococci and thus cannot be recommended for routine use.

Original languageEnglish (US)
Pages (from-to)1675-1678
Number of pages4
JournalJournal of clinical microbiology
Issue number9
StatePublished - 1988

ASJC Scopus subject areas

  • Microbiology (medical)


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