@article{34a8379f302a4a9da196c2d0a05b71bb,
title = "Salivary polypeptide/hyaluronic acid multilayer coatings act as {"}fungal repellents{"} and prevent biofilm formation on biomaterials",
abstract = "Candida-associated denture stomatitis (CADS) is a common, recurring clinical complication in denture wearers that can lead to serious oral and systemic health problems. Current management strategies are not satisfactory due to their short-acting and ineffective therapeutic effects. Here, we describe a new fungal biofilm controlling strategy using the polyelectrolyte layer-by-layer (LBL) self-assembly technology on denture materials. Conventional poly(methyl methacrylate) (PMMA) denture material discs were functionalized with negatively charged poly(methacrylic acid) (PMAA) via plasma-initiated surface grafting, followed by repetitive alternating coating with the salivary antimicrobial polypeptide histatin 5 (H-5; cationic polymer) and hyaluronic acid (HA; anionic polymer). On the other hand, the H-5/HA LBL coatings (i.e., the outermost layer was H-5) inhibited fungal attachment/adhesion, significantly reduced fungal biofilm formation, and showed synergistic effects with the antifungal drug miconazole. LBL surface hydrophilicity was not the key mechanism in controlling Candida biofilm formation. The current approach demonstrates the utility of a new design principle for fabricating anticandidal denture materials, as well as potentially other related medical devices, for controlling fungal biofilm formation and combating insidious infections.",
author = "Jianchuan Wen and Yeh, {Chih Ko} and Yuyu Sun",
note = "Funding Information: We are grateful to Dr. David D. Dean (Professor, Comprehensive Dentistry, UTHSCSA) for his critical review and editing of the manuscript. This study was supported by the National Institutes of Health (NIH), NIDCR (R01 DE021084) and VA Merit Review (I01BX001103). Funding Information: In summary, we demonstrated that the fungal biofilm-controlling functionality can be introduced onto PMMA-based denture materials by grafting PMAA onto the surface, followed by H-5/HA bilayer coating using the LBL self-assembly technology. We found that the hydrophilic surface alone was ineffective in controlling Candida biofilm formation. Although H-5 in the multilayer coatings did not provide fungicidal activity in the direct contact anticandidal tests, its antifungal activity significantly reduced the initial adhesion of fungal cells and effectively prevented biofilm formation. Thus, H-5 in the coatings acted as a {\textquoteleft}{\textquoteleft}fungal repellent{\textquoteright}{\textquoteright} to control Candida colonization and biofilm formation. Furthermore, the anti-adhesion and We are grateful to Dr. David D. Dean (Professor, Comprehensive Dentistry, UTHSCSA) for his critical review and editing of the manuscript. This study was supported by the National Institutes of Health (NIH), NIDCR (R01 DE021084) and VA Merit Review (I01BX001103). Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",
year = "2018",
month = mar,
day = "14",
doi = "10.1039/c7tb02592k",
language = "English (US)",
volume = "6",
pages = "1452--1457",
journal = "Journal of Materials Chemistry B",
issn = "2050-7518",
publisher = "Royal Society of Chemistry",
number = "10",
}