Salivary alterations in type 2 (non-insulin-dependent) diabetes mellitus and hypertension

Michael W J Dodds, Chih-ko Yeh, Dorthea A. Johnson

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Objectives: The aim of this study was to determine whether saliva output and composition are altered in type 2 diabetes mellitus by comparison with a healthy, non-medicated control group, and also a group of hypertensives. Methods: From a community-dwelling cohort of Mexican American and European American subjects enrolled in the OH:SALSA oral aging study, we identified 233 subjects with type 2 diabetes mellitus, 227 with hypertension, and 240 healthy control subjects. We collected unstimulated whole (UW) and submandibular/ sublingual (US) saliva, as well as stimulated parotid (SP) and submandibular/ sublingual (SS) saliva. Flow rates were determined, yeast carriage was assayed in UW saliva, and SP and SS saliva samples were analyzed for protein composition. ELISA was used to determine concentrations of an array of specific protein components, with both antimicrobial and other activities. Results: Both diabetic and hypertensive subjects had reduced output of both stimulated and unstimulated submandibular/sublingual saliva. 30% of the diabetic subjects had high oral yeast counts (≥1000 cfu/mL) compared with 17% of the healthy subjects and 20% of the hypertensives. Significant increases in the concentrations of a number of the protein components were found in the diabetic subjects, specifically, SP lactoferrin, myeloperoxidase (MPO), and salivary peroxidase (SPO), as well as SS total protein, albumin, lactoferrin and secretory IgA. Conclusions: The pattern of decreased flow rates and increased protein concentrations were similar, but consistently greater in diabetics than hypertensives, suggesting that disease-specific mechanisms may be responsible. Diabetics may be more prone to oral dryness and infections than non-diabetics.

Original languageEnglish (US)
Pages (from-to)373-381
Number of pages9
JournalCommunity Dentistry and Oral Epidemiology
Volume28
Issue number5
StatePublished - 2000

Fingerprint

Saliva
Type 2 Diabetes Mellitus
Hypertension
Lactoferrin
Peroxidase
Healthy Volunteers
Proteins
Yeasts
Independent Living
Secretory Immunoglobulin A
Xerostomia
Protein Array Analysis
Albumins
Enzyme-Linked Immunosorbent Assay
Control Groups
Infection

Keywords

  • Diabetes mellitus
  • Hypertension
  • Non-insulin- Dependent
  • Saliva
  • Salivary glands
  • Salivary proteins

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Salivary alterations in type 2 (non-insulin-dependent) diabetes mellitus and hypertension. / Dodds, Michael W J; Yeh, Chih-ko; Johnson, Dorthea A.

In: Community Dentistry and Oral Epidemiology, Vol. 28, No. 5, 2000, p. 373-381.

Research output: Contribution to journalArticle

@article{ecbc29a302e8435cbf9499f6f11c2584,
title = "Salivary alterations in type 2 (non-insulin-dependent) diabetes mellitus and hypertension",
abstract = "Objectives: The aim of this study was to determine whether saliva output and composition are altered in type 2 diabetes mellitus by comparison with a healthy, non-medicated control group, and also a group of hypertensives. Methods: From a community-dwelling cohort of Mexican American and European American subjects enrolled in the OH:SALSA oral aging study, we identified 233 subjects with type 2 diabetes mellitus, 227 with hypertension, and 240 healthy control subjects. We collected unstimulated whole (UW) and submandibular/ sublingual (US) saliva, as well as stimulated parotid (SP) and submandibular/ sublingual (SS) saliva. Flow rates were determined, yeast carriage was assayed in UW saliva, and SP and SS saliva samples were analyzed for protein composition. ELISA was used to determine concentrations of an array of specific protein components, with both antimicrobial and other activities. Results: Both diabetic and hypertensive subjects had reduced output of both stimulated and unstimulated submandibular/sublingual saliva. 30{\%} of the diabetic subjects had high oral yeast counts (≥1000 cfu/mL) compared with 17{\%} of the healthy subjects and 20{\%} of the hypertensives. Significant increases in the concentrations of a number of the protein components were found in the diabetic subjects, specifically, SP lactoferrin, myeloperoxidase (MPO), and salivary peroxidase (SPO), as well as SS total protein, albumin, lactoferrin and secretory IgA. Conclusions: The pattern of decreased flow rates and increased protein concentrations were similar, but consistently greater in diabetics than hypertensives, suggesting that disease-specific mechanisms may be responsible. Diabetics may be more prone to oral dryness and infections than non-diabetics.",
keywords = "Diabetes mellitus, Hypertension, Non-insulin- Dependent, Saliva, Salivary glands, Salivary proteins",
author = "Dodds, {Michael W J} and Chih-ko Yeh and Johnson, {Dorthea A.}",
year = "2000",
language = "English (US)",
volume = "28",
pages = "373--381",
journal = "Community Dentistry and Oral Epidemiology",
issn = "0301-5661",
publisher = "Blackwell Munksgaard",
number = "5",

}

TY - JOUR

T1 - Salivary alterations in type 2 (non-insulin-dependent) diabetes mellitus and hypertension

AU - Dodds, Michael W J

AU - Yeh, Chih-ko

AU - Johnson, Dorthea A.

PY - 2000

Y1 - 2000

N2 - Objectives: The aim of this study was to determine whether saliva output and composition are altered in type 2 diabetes mellitus by comparison with a healthy, non-medicated control group, and also a group of hypertensives. Methods: From a community-dwelling cohort of Mexican American and European American subjects enrolled in the OH:SALSA oral aging study, we identified 233 subjects with type 2 diabetes mellitus, 227 with hypertension, and 240 healthy control subjects. We collected unstimulated whole (UW) and submandibular/ sublingual (US) saliva, as well as stimulated parotid (SP) and submandibular/ sublingual (SS) saliva. Flow rates were determined, yeast carriage was assayed in UW saliva, and SP and SS saliva samples were analyzed for protein composition. ELISA was used to determine concentrations of an array of specific protein components, with both antimicrobial and other activities. Results: Both diabetic and hypertensive subjects had reduced output of both stimulated and unstimulated submandibular/sublingual saliva. 30% of the diabetic subjects had high oral yeast counts (≥1000 cfu/mL) compared with 17% of the healthy subjects and 20% of the hypertensives. Significant increases in the concentrations of a number of the protein components were found in the diabetic subjects, specifically, SP lactoferrin, myeloperoxidase (MPO), and salivary peroxidase (SPO), as well as SS total protein, albumin, lactoferrin and secretory IgA. Conclusions: The pattern of decreased flow rates and increased protein concentrations were similar, but consistently greater in diabetics than hypertensives, suggesting that disease-specific mechanisms may be responsible. Diabetics may be more prone to oral dryness and infections than non-diabetics.

AB - Objectives: The aim of this study was to determine whether saliva output and composition are altered in type 2 diabetes mellitus by comparison with a healthy, non-medicated control group, and also a group of hypertensives. Methods: From a community-dwelling cohort of Mexican American and European American subjects enrolled in the OH:SALSA oral aging study, we identified 233 subjects with type 2 diabetes mellitus, 227 with hypertension, and 240 healthy control subjects. We collected unstimulated whole (UW) and submandibular/ sublingual (US) saliva, as well as stimulated parotid (SP) and submandibular/ sublingual (SS) saliva. Flow rates were determined, yeast carriage was assayed in UW saliva, and SP and SS saliva samples were analyzed for protein composition. ELISA was used to determine concentrations of an array of specific protein components, with both antimicrobial and other activities. Results: Both diabetic and hypertensive subjects had reduced output of both stimulated and unstimulated submandibular/sublingual saliva. 30% of the diabetic subjects had high oral yeast counts (≥1000 cfu/mL) compared with 17% of the healthy subjects and 20% of the hypertensives. Significant increases in the concentrations of a number of the protein components were found in the diabetic subjects, specifically, SP lactoferrin, myeloperoxidase (MPO), and salivary peroxidase (SPO), as well as SS total protein, albumin, lactoferrin and secretory IgA. Conclusions: The pattern of decreased flow rates and increased protein concentrations were similar, but consistently greater in diabetics than hypertensives, suggesting that disease-specific mechanisms may be responsible. Diabetics may be more prone to oral dryness and infections than non-diabetics.

KW - Diabetes mellitus

KW - Hypertension

KW - Non-insulin- Dependent

KW - Saliva

KW - Salivary glands

KW - Salivary proteins

UR - http://www.scopus.com/inward/record.url?scp=0034292801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034292801&partnerID=8YFLogxK

M3 - Article

C2 - 11014514

AN - SCOPUS:0034292801

VL - 28

SP - 373

EP - 381

JO - Community Dentistry and Oral Epidemiology

JF - Community Dentistry and Oral Epidemiology

SN - 0301-5661

IS - 5

ER -