Saccharomyces Ku70, Mre11/Rad50, and RPA proteins regulate adaptation to G2/M arrest after DNA damage

Sang Lee, J. Kent Moore, Allyson Holmes, Keiko Umezu, Richard D. Kolodner, James E. Haber

Research output: Contribution to journalArticle

591 Citations (Scopus)

Abstract

Saccharomyces cells suffering a single unrepairable double-strand break (DSB) exhibit a long, but transient arrest at G2/M. hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint. The effect of hdf1 results from its accelerated 5' to 3' degradation of the broken chromosome. Permanent arrest in hdf1 cells is suppressed by rad50 or mre11 deletions that retard this degradation. Wild-type HDF1 cells also become permanently arrested when they experience two unrepairable DSBs. Both DSB- induced arrest conditions are suppressed by a mutation in the single-strand binding protein, RPA. We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation.

Original languageEnglish (US)
Pages (from-to)399-409
Number of pages11
JournalCell
Volume94
Issue number3
DOIs
StatePublished - Aug 7 1998
Externally publishedYes

Fingerprint

Saccharomyces
Chromosomes
DNA Damage
Degradation
DNA
Carrier Proteins
Proteins
Mutation
1,2-di-(4-sulfamidophenyl)-4-butylpyrazolidine-3,5-dione

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Saccharomyces Ku70, Mre11/Rad50, and RPA proteins regulate adaptation to G2/M arrest after DNA damage. / Lee, Sang; Moore, J. Kent; Holmes, Allyson; Umezu, Keiko; Kolodner, Richard D.; Haber, James E.

In: Cell, Vol. 94, No. 3, 07.08.1998, p. 399-409.

Research output: Contribution to journalArticle

Lee, Sang ; Moore, J. Kent ; Holmes, Allyson ; Umezu, Keiko ; Kolodner, Richard D. ; Haber, James E. / Saccharomyces Ku70, Mre11/Rad50, and RPA proteins regulate adaptation to G2/M arrest after DNA damage. In: Cell. 1998 ; Vol. 94, No. 3. pp. 399-409.
@article{0ab867fa505445e8af3d416158f2c9fb,
title = "Saccharomyces Ku70, Mre11/Rad50, and RPA proteins regulate adaptation to G2/M arrest after DNA damage",
abstract = "Saccharomyces cells suffering a single unrepairable double-strand break (DSB) exhibit a long, but transient arrest at G2/M. hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint. The effect of hdf1 results from its accelerated 5' to 3' degradation of the broken chromosome. Permanent arrest in hdf1 cells is suppressed by rad50 or mre11 deletions that retard this degradation. Wild-type HDF1 cells also become permanently arrested when they experience two unrepairable DSBs. Both DSB- induced arrest conditions are suppressed by a mutation in the single-strand binding protein, RPA. We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation.",
author = "Sang Lee and Moore, {J. Kent} and Allyson Holmes and Keiko Umezu and Kolodner, {Richard D.} and Haber, {James E.}",
year = "1998",
month = "8",
day = "7",
doi = "10.1016/S0092-8674(00)81482-8",
language = "English (US)",
volume = "94",
pages = "399--409",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - Saccharomyces Ku70, Mre11/Rad50, and RPA proteins regulate adaptation to G2/M arrest after DNA damage

AU - Lee, Sang

AU - Moore, J. Kent

AU - Holmes, Allyson

AU - Umezu, Keiko

AU - Kolodner, Richard D.

AU - Haber, James E.

PY - 1998/8/7

Y1 - 1998/8/7

N2 - Saccharomyces cells suffering a single unrepairable double-strand break (DSB) exhibit a long, but transient arrest at G2/M. hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint. The effect of hdf1 results from its accelerated 5' to 3' degradation of the broken chromosome. Permanent arrest in hdf1 cells is suppressed by rad50 or mre11 deletions that retard this degradation. Wild-type HDF1 cells also become permanently arrested when they experience two unrepairable DSBs. Both DSB- induced arrest conditions are suppressed by a mutation in the single-strand binding protein, RPA. We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation.

AB - Saccharomyces cells suffering a single unrepairable double-strand break (DSB) exhibit a long, but transient arrest at G2/M. hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint. The effect of hdf1 results from its accelerated 5' to 3' degradation of the broken chromosome. Permanent arrest in hdf1 cells is suppressed by rad50 or mre11 deletions that retard this degradation. Wild-type HDF1 cells also become permanently arrested when they experience two unrepairable DSBs. Both DSB- induced arrest conditions are suppressed by a mutation in the single-strand binding protein, RPA. We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation.

UR - http://www.scopus.com/inward/record.url?scp=0032493889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032493889&partnerID=8YFLogxK

U2 - 10.1016/S0092-8674(00)81482-8

DO - 10.1016/S0092-8674(00)81482-8

M3 - Article

C2 - 9708741

AN - SCOPUS:0032493889

VL - 94

SP - 399

EP - 409

JO - Cell

JF - Cell

SN - 0092-8674

IS - 3

ER -