Saccharomyces cerevisiae ATM orthologue suppresses break-induced chromosome translocations

Kihoon Lee, Yu Zhang, Sang Eun Lee

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Chromosome translocations are frequently associated with many types of blood-related cancers and childhood sarcomas. Detection of chromosome translocations assists in diagnosis, treatment and prognosis of these diseases; however, despite their importance to such diseases, the molecular mechanisms leading to chromosome translocations are not well understood. The available evidence indicates a role for non-homologous end joining (NHEJ) of DNA double-strand breaks (DSBs) in their origin. Here we develop a yeast-based system that induces a reciprocal chromosome translocation by formation and ligation of breaks on two different chromosomes. We show that interchromosomal end joining is efficiently suppressed by the Tel1- and Mre11-Rad50-Xrs2- dependent pathway; this is distinct from the role of Tel1 in telomeric integrity and from Mec1- and Tel1-dependent checkpoint controls. Suppression of DSB-induced chromosome translocations depends on the kinase activity of Tel1 and Dun1, and the damage-induced phosphorylation of Sae2 and histone H2AX proteins. Tel1- and Sae2-dependent tethering and promotion of 5′ to 3′ degradation of broken chromosome ends discourage error-prone NHEJ and interchromosomal NHEJ, preserving chromosome integrity on DNA damage. Our results indicate that, like human ATM, Tel1 serves as a key regulator for chromosome integrity in the pathway that reduces the risk for DSB-induced chromosome translocations, and are probably pertinent to the oncogenic chromosome translocations in ATM-deficient cells.

Original languageEnglish (US)
Pages (from-to)543-546
Number of pages4
Issue number7203
StatePublished - Jul 24 2008

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Saccharomyces cerevisiae ATM orthologue suppresses break-induced chromosome translocations'. Together they form a unique fingerprint.

Cite this