RSC mobilizes nucleosomes to improve accessibility of repair machinery to the damaged chromatin

Eun Yong Shim, Soo Jin Hong, Ji Hyun Oum, Yvonne Yanez, Yu Zhang, Sang Eun Lee

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

Repair of DNA double-strand breaks (DSBs) protects cells and organisms, as well as their genome integrity. Since DSB repair occurs in the context of chroma tin, chromatin must be modified to prevent it from inhibiting DSB repair. Evidence supports the role of histone modifications and ATP-dependent chromatin remodeling in repair and signaling of chromosome DSBs. The key questions are, then, what the nature of chromatin altered by DSBs is and how remodeling of chromatin facilitates DSB repair. Here we report a chromatin alteration caused by a single HO endonuclease-generated DSB at the Saccharomyces cerevisiae MAT locus. The break induces rapid nucleosome migration to form histone-free DNA of a few hundred base pairs immediately adjacent to the break. The DSB-induced nucleosome repositioning appears independent of end processing, since it still occurs when the 5′-to-3′ degradation of the DNA end is markedly reduced. The tetracycline-controlled depletion of Sth1, the ATPase of RSC, or deletion of RSC2 severely reduces chromatin remodeling and loading of Mre11 and Yku proteins at the DSB. Depletion of Sth1 also reduces phosphorylation of H2A, processing, and joining of DSBs. We propose that RSC-mediated chromatin remodeling at the DSB prepares chromatin to allow repair machinery to access the break and is vital for efficient DSB repair.

Original languageEnglish (US)
Pages (from-to)1602-1613
Number of pages12
JournalMolecular and cellular biology
Volume27
Issue number5
DOIs
StatePublished - Mar 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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