Roles of NPM2 in chromatin and nucleolar organization in oocytes and embryos

Kathleen H. Burns, Maria M. Viveiros, Yongsheng Ren, Pei Wang, Francesco J. DeMayo, Donald E. Frail, John J. Eppig, Martin M. Matzuk

Research output: Contribution to journalArticle

272 Scopus citations

Abstract

Upon fertilization, remodeling of condensed maternal and paternal gamete DNA occurs to form the diploid genome. In Xenopus laevis, nucleoplasmin 2 (NPM2) decondenses sperm DNA in vitro. To study chromatin remodeling in vivo, we isolated mammalian NPM2 orthologs. Mouse NPM2 accumulates in oocyte nuclei and persists in preimplantation embryos. Npm2 knockout females have fertility defects owing to failed preimplantation embryo development. Although sperm DNA decondensation proceeds without NPH2, abnormalities are evident in oocyte and early embryonic nuclei. These defects include an absence of coalesced nucleolar structures and loss of heterochromatin and deacetylated histone H3 that normally circumscribe nucleoli in oocytes and early embryos, respectively. Thus, Npm2 is a maternal effect gene critical for nuclear and nucleolar organization and embryonic development.

Original languageEnglish (US)
Pages (from-to)633-636
Number of pages4
JournalScience
Volume300
Issue number5619
DOIs
StatePublished - Apr 25 2003
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Roles of NPM2 in chromatin and nucleolar organization in oocytes and embryos'. Together they form a unique fingerprint.

  • Cite this

    Burns, K. H., Viveiros, M. M., Ren, Y., Wang, P., DeMayo, F. J., Frail, D. E., Eppig, J. J., & Matzuk, M. M. (2003). Roles of NPM2 in chromatin and nucleolar organization in oocytes and embryos. Science, 300(5619), 633-636. https://doi.org/10.1126/science.1081813